Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model.
Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model.
复制标题
兔前十字韧带横断模型中骨关节炎进展过程中软骨下骨的血管生成活性。
DOI：
10.1016/j.joca.2012.08.023
复制
发表时间：
2012
影响因子：
7
通讯作者：
Kazuhisa Takahashi
中科院分区：
文献类型：
--
作者：
M. Saito;T. Sasho;S. Yamaguchi;N. Ikegawa;R. Akagi;Y. Muramatsu;S. Mukoyama;N. Ochiai;J. Nakamura;K. Nakagawa;A. Nakajima;Kazuhisa Takahashi
目的建立兔骨关节炎（OA）模型，观察软骨下骨和软骨在骨性关节炎（OA）进展过程中的纵向血管生成活性。材料与方法采用12月龄新西兰大白兔左膝前交叉韧带置换术（ACLT）诱导soa。于ACLT后0、2、4、6、8、12周进行组织学检查、免疫组化和血管生成活性测定。采用血红素染色、伊红染色、红花素- o染色观察股骨内侧髁（MFC）、外侧髁（LFC）骨关节炎变化。CD31免疫组化证实骨软骨连接处血管浸润。采用共培养小管形成试验评价MFC和LFC软骨下骨和软骨以及滑膜的血管生成活性。评估组织学改变、血管生成活性和血管侵犯之间的关系。结果soa变化在MFC和LFC中都以时间依赖的方式增加。软骨下骨血管生成活性在骨性关节炎进展过程中呈单峰变化，在早期至进展期达到峰值，在骨性关节炎晚期降至正常水平。在OA的进展阶段，血管生成活性增加，血管侵入也随之增加。软骨血管生成活性在骨性关节炎进展过程中没有改变。结论骨性关节炎早期至进展期软骨下骨血管生成活性升高，血管侵入骨软骨连接处。
OBJECTIVETo investigate the longitudinal angiogenic activity of subchondral bone and cartilage during the progression of osteoarthritis (OA) using a rabbit model of OA.MATERIALS AND METHODSOA was surgically induced by anterior cruciate ligament transaction (ACLT) in left knee of 12 months old female New Zealand white rabbits (n = 33). Histological examination, immunohistochemistry, and angiogenic activity assay was done at 0, 2, 4, 6, 8, 12 weeks after ACLT. Histologic evaluation was performed with haematoxylin and eosin, safranin-O staining to assess the OA change of medial femoral condyle (MFC) and lateral femoral condyle (LFC). CD31 immunohistochemistry was performed to confirm the vascular invasion at osteochondral junction. A co-cultured tubule formation assay was conducted to evaluate angiogenic activity of the subchondral bone and cartilage of MFC and LFC as well as synovium. Association between histological changes, angiogenic activity, and vascular invasion were evaluated.RESULTSOA changes increased in a time-dependent manner both in MFC and LFC. Angiogenic activity of subchondral bone showed a monomodal change during the OA progression, achieved a peak in the early to progressive stage and decreased to normal level in the late stage of OA. Surge of vascular invasion was observed following the increase of angiogenic activity in the progressive stage of OA. Angiogenic activity of cartilage did not change during the course of OA progression.CONCLUSIONAngiogenic activity of subchondral bone was elevated in the early to progressive stage of OA and vascular invasion into the osteochondral junction followed.
影响因子：
7
作者：
Yoshioka, M;Coutts, RD;Hacker, SA
通讯作者：
Hacker, SA