Interleukin enhancer-binding factor 3 and HOXC8 co-activate cadherin 11 transcription to promote breast cancer cells proliferation and migration.
Interleukin enhancer-binding factor 3 and HOXC8 co-activate cadherin 11 transcription to promote breast cancer cells proliferation and migration.
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白细胞介素增强子结合因子3和HOXC8共同激活钙粘蛋白11转录促进乳腺癌细胞增殖和迁移
DOI：
10.18632/oncotarget.22491
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发表时间：
2017-12-08
期刊：
影响因子：
--
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Zhang Y;Yang C;Zhang M;Liu H;Gong C;Zhang J;Xu S;Zou J;Kai Y;Li Y
钙粘蛋白11（CDH 11）的表达仅在浸润性乳腺癌细胞和侵袭性乳腺癌标本中检测到。然而，很少有人知道CDH 11转录调控的分子机制。在这里，我们报告，白细胞介素增强子结合因子3（ILF 3）与同源框C8（HOXC 8）相互作用，激活乳腺癌细胞中的CDH 11转录。使用免疫共沉淀和质谱分析，显示ILF 3与乳腺癌细胞中的HOXC 8相互作用。我们证明ILF 3与CDH 11启动子的-2982 ~ -2978和-2602 ~2598核苷酸结合，并与HOXC 8相互作用，共同激活CDH 11的转录。我们进一步表明，ILF 3促进增殖和迁移，至少部分地，通过促进乳腺癌细胞中的CDH 11表达。此外，免疫组织化学（IHC）显示，与正常乳腺组织相比，乳腺癌标本中CDH 11、ILF 3和HOXC 8的表达均上调。重要的是，CDH 11、ILF 3和HOXC 8的表达水平在乳腺癌的晚期阶段升高，并且CDH 11、ILF 3和HOXC 8的高表达与乳腺癌患者的不良无远处转移生存（DMFS）相关。
Cadherin 11 (CDH11) expression is detected only in invasive breast cancer cells and aggressive breast cancer specimens. However, little is known about the molecular mechanisms of CDH11 transcriptional regulation. Here, we report that interleukin enhancer binding factor 3 (ILF3) interacts with Homeobox C8 (HOXC8) to activate CDH11 transcription in breast cancer cells. Using co-immunoprecipitation and mass spectrometry analyses, ILF3 is shown to interact with HOXC8 in breast cancer cells. We demonstrate that ILF3 binds to the CDH11 promoter on nucleotides –2982 ~ –2978 and –2602 ~ 2598 and interacts with HOXC8 to co-activate CDH11 transcription. We further show that ILF3 promotes proliferation and migration, at least partially, by facilitating CDH11 expression in breast cancer cells. Moreover, immunohistochemistry (IHC) shows that expression of CDH11, ILF3 and HOXC8 are all upregulated in breast cancer specimens compared to normal breast tissues. Importantly, the expression levels of CDH11, ILF3 and HOXC8 are elevated in the advanced stages of breast cancer, and high expression of CDH11, ILF3 and HOXC8 is associated with poor distant metastasis-free survival (DMFS) for breast cancer patients.
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
4.8
作者：
Kiesler, Patricia;Haynes, Paul A.;Vercelli, Donata
通讯作者：
Vercelli, Donata
影响因子：
5.3
作者：
Kuwano, Yuki;Kim, Hyeon Ho;Gorospe, Myriam
通讯作者：
Gorospe, Myriam
DOI：
10.1073/pnas.0409700102
发表时间：
2005-02-15
影响因子：
11.1
作者：
Lei, HY;Wang, HL;Ruddle, FH
通讯作者：
Ruddle, FH
HOXC8通过转录促进cadherin-11表达促进乳腺肿瘤发生
影响因子：
--
作者：
Li, Yong;Chao, Fengmei;Huang, Shuang
通讯作者：
Huang, Shuang