Mono-cationic detergents play a critical role in the development of liposome-based gene vector via controlling its lamellarity
Mono-cationic detergents play a critical role in the development of liposome-based gene vector via controlling its lamellarity
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单阳离子去垢剂通过控制其层状在基于脂质体的基因载体的开发中发挥关键作用
DOI：
10.1007/s11051-013-2227-8
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发表时间：
2014
影响因子：
2.5
通讯作者：
H. Harashima
中科院分区：
文献类型：
--
作者：
Ryosuke Suzuki;Yuma Yamada;E. Kawamura;H. Harashima
在脂质体中，控制脂质双分子层的数量，即层状结构，是使用基于脂质体的纳米载体给药的体内/体外药代动力学的一个主要因素。先前的研究结果表明，单阳离子洗涤剂（MCD）可以通过与脂质包膜的相互作用来控制脂质体的大小。本研究利用多功能包膜型纳米装置（MEND）研究了MCD控制脂质体基因载体的片层性。MEND由浓缩质粒DNA核和脂质包膜组成。含MCD的MEND （MCD-MEND）的大小随MCD含量的增加而减小，表明MCD可用于控制脂质双分子层的数量。我们还通过将双层MEND包装到含mcd的脂质双分子层中，开发了三层MEND （TL-MEND）。我们假设TL-MEND可以有效地将基因传递到细胞核，当外单层双膜与质膜融合，内双层膜与核双层膜融合。转染实验表明，TL-MEND在JAWS II细胞（非分裂细胞）中具有较高的转染活性。这些结果表明，MCD具有通过控制脂质体层状结构来增强基因传递的潜力。
Controlling the number of lipid bilayers, the lamellarity, in a liposome is a major factor in the in vivo/in vitro pharmacokinetics of drug delivery using liposome-based nanocarriers. Findings reported in a previous study indicated that a mono-cationic detergent (MCD) could be useful in controlling liposomal size via interaction with the lipid envelope. Here, we investigated controlling the lamellarity of the liposomal gene vector by MCD, using a multifunctional envelope-type nano device (MEND). The MEND consisted of a condensed plasmid DNA core and lipid envelopes. The size of the MCD-contained MEND (MCD-MEND) decreased as a function of the amount of MCD, indicating that MCD can be used to control the number of the lipid bilayers. We also developed a triple-layered MEND (TL-MEND) by packaging a di-lamellar MEND into an MCD-containing lipid bilayer. We hypothesized that the TL-MEND would efficiently deliver a gene to the nucleus, when the outer single bilayer fused with the plasma membrane and the inner double membranes then fused with the nuclear double membranes. Transfection assays showed that the TL-MEND had a high transfection activity in JAWS II cells, non-dividing cells. These results indicate that MCD has the potential for enhancing the gene delivery by controlling liposomal lamellarity.
影响因子：
2.9
作者：
MIMMS, LT;ZAMPIGHI, G;REYNOLDS, JA
通讯作者：
REYNOLDS, JA
影响因子：
2.9
作者：
MCINTYRE, JC;SLEIGHT, RG
通讯作者：
SLEIGHT, RG
影响因子：
46.2
作者：
Kamaly N;Xiao Z;Valencia PM;Radovic-Moreno AF;Farokhzad OC
通讯作者：
Farokhzad OC