Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain.
Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain.
复制标题
DOI：
10.1186/s12917-018-1451-y
复制
发表时间：
2018-04-19
影响因子：
2.6
通讯作者：
Schmoll F
中科院分区：
文献类型：
--
作者：
Sattler T;Pikalo J;Wodak E;Revilla-Fernández S;Steinrigl A;Bagó Z;Entenfellner F;Claude JB;Pez F;Francillette M;Schmoll F
针对猪繁殖与呼吸综合征病毒（PRRSV），特别是针对高致病性（HP）PRRSV的有效疫苗仍然缺乏。本研究的目的是评估由两种毒株组成的实验性PRRSV 2减毒活疫苗对越南HP PRRSV 2野毒株的异源攻击的保护效力。为此，将20头PRRSV阴性仔猪分为两组。第1组的猪接种实验疫苗，第2组保持未接种。所有研究仔猪在研究第0天（接种后42天）接受HP PRRSV 2鼻内攻毒。在疫苗接种后第7天和第21天以及攻毒后几天采集血液样本。攻毒后第28天，对所有仔猪实施安乐死并进行病理学检查。在接种后第7天和第21天，在第1组的所有仔猪中观察到PRRSV 2型病毒血症，在接种后42天内，在7头仔猪中仍可检出。攻毒后第3天，两组仔猪的PRRSV 2 RT-qPCR均为阳性。从第7天开始，组1中的病毒载量和PRRSV 2阳性猪的数量低于组2。组1的所有猪在PRRSV 2疫苗接种后发生血清转化。从攻毒后第14天开始，在两组所有研究猪的血清中检测到PRRSV抗体。在第2组中，在HP PRRSV 2攻毒后观察到中度呼吸道症状伴偶尔咳嗽。第1组的猪在临床上未受影响。在组1的4头仔猪和组2的所有10头仔猪中发现间质性肺炎。第2组的组织学结果更严重。因此得出结论，所用的PRRSV 2活实验疫苗可预防临床疾病，并显著降低越南HP PRRSV 2田间毒株攻毒猪的组织病理学结果和病毒载量。
Effective vaccines against porcine reproductive and respiratory syndrome virus (PRRSV), especially against highly pathogenic (HP) PRRSV are still missing. The objective of this study was to evaluate the protective efficacy of an experimental live attenuated PRRSV 2 vaccine, composed of two strains, against heterologous challenge with a Vietnamese HP PRRSV 2 field strain. For this reason, 20 PRRSV negative piglets were divided into two groups. The pigs of group 1 were vaccinated with the experimental vaccine, group 2 remained unvaccinated. All study piglets received an intranasal challenge of the HP PRRSV 2 on day 0 of the study (42 days after vaccination). Blood samples were taken on days 7 and 21 after vaccination and on several days after challenge. On day 28 after challenge, all piglets were euthanized and pathologically examined. On days 7 and 21 after vaccination, a PRRSV 2 viraemia was seen in all piglets of group 1 which remained detectable in seven piglets up to 42 days after vaccination. On day 3 after challenge, all piglets from both groups were positive in PRRSV 2 RT-qPCR. From day 7 onwards, viral load and number of PRRSV 2 positive pigs were lower in group 1 than in group 2. All pigs of group 1 seroconverted after PRRSV 2 vaccination. PRRSV antibodies were detected in serum of all study pigs from both groups from day 14 after challenge onwards. In group 2, moderate respiratory symptoms with occasional coughing were seen following the challenge with HP PRRSV 2. Pigs of group 1 remained clinically unaffected. Interstitial pneumonia was found in four piglets of group 1 and in all ten piglets of group 2. Histopathological findings were more severe in group 2. It was thus concluded that the used PRRSV 2 live experimental vaccine provided protection from clinical disease and marked reduction of histopathological findings and viral load in pigs challenged with a Vietnamese HP PRRSV 2 field strain.
登录
查看更多内容
猪呼吸与繁殖综合征病毒变种，越南和中国，2007 年。
影响因子：
11.8
作者：
Feng Y;Zhao T;Nguyen T;Inui K;Ma Y;Nguyen TH;Nguyen VC;Liu D;Bui QA;To LT;Wang C;Tian K;Gao GF
通讯作者：
Gao GF
影响因子：
1.6
作者：
Li, Yan;Xue, Chunyi;Cao, Yongchang
通讯作者：
Cao, Yongchang
影响因子：
5.5
作者：
Martelli, Paolo;Gozio, Stefano;Borghetti, Paolo
通讯作者：
Borghetti, Paolo
影响因子：
3.3
作者：
Stadejek, Tomasz;Stankevicius, Arunas;Oleksiewicz, Martin B.
通讯作者：
Oleksiewicz, Martin B.
影响因子：
2.7
作者：
Adams MJ;Lefkowitz EJ;King AM;Harrach B;Harrison RL;Knowles NJ;Kropinski AM;Krupovic M;Kuhn JH;Mushegian AR;Nibert M;Sabanadzovic S;Sanfaçon H;Siddell SG;Simmonds P;Varsani A;Zerbini FM;Gorbalenya AE;Davison AJ
通讯作者：
Davison AJ