Comparison of Metabolic Alterations in Serum and Milk Whey Between Inactive Ovaries and Estrus Dairy Cows.
Comparison of Metabolic Alterations in Serum and Milk Whey Between Inactive Ovaries and Estrus Dairy Cows.
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卵巢不活跃与发情奶牛血清和乳清代谢变化比较
DOI：
10.3389/fvets.2020.609391
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发表时间：
2020
影响因子：
3.2
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Zhao C;Bai Y;Fu S;Wu L;Xia C;Xu C
卵巢失活影响奶牛泌乳早期的发情周期和定时人工授精（TAI）效率。本试验的目的是确定奶牛IO和发情期血清和乳清代谢的变化。在产后30天选择28头年龄、产奶量和身体状况相似的健康产后荷斯坦奶牛，跟踪至产后70天，并通过Afi Farm®软件记录发情性能。由有经验的饲养员通过B超和直肠检查对奶牛的卵巢状况和卵泡直径进行了检查。对照组为正常发情母牛14头，IO组为B母牛14头，均在产后30-70 d。采用非靶向核磁共振（1H-NMR）技术，对两组奶牛产后70 d的血清和乳清进行分析，测定IO奶牛的不同代谢产物。与A组相比，B组产后70 d时，琥珀酸、磷酸肌酸、甘氨酸、肌醇、乙醇酸、乳清酸等6种代谢产物的乳清含量增加，丙氨酸、肌酐、O-磷酸胆碱、乳糖、牛磺酸、半乳糖、葡萄糖-1-磷酸等7种代谢产物的乳清含量减少。与A组相比，B组奶牛血清中4种差异代谢物（包括3-羟基丁酸、乙酸、谷氨酰胺和甘氨酸）增加，9种差异代谢物（包括丙氨酸、琥珀酸、柠檬酸、肌酐、o-磷酸胆碱、葡萄糖、肌醇、酪氨酸和组氨酸）减少。B组IO奶牛葡萄糖代谢降低，三羧酸循环受损，脂质动员增加，氨基酸代谢异常。本研究为今后奶牛IO的预防提供了一种可能的策略。
Inactive ovaries (IOs) affect the estrus cycle and timed artificial insemination (TAI) efficiency in dairy cows during early lactation. The objective of the experiment was to determine metabolic changes in the serum and milk whey of dairy cows with IO and estrus. Twenty-eight healthy postpartum Holstein cows in similar age, milk production, and body condition were selected at 30 days postpartum for tracking to 70 days postpartum, and estrus performance was recorded through Afi Farm® software. The ovarian status and follicular diameter of dairy cows were examined by an experienced breeder through B-ultrasound and rectal examination. Fourteen normal estrus cows were allocated to control group A and 14 cows with IO to group B, all at 30–70 days postpartum. The serum and milk whey in the two groups of cows at 70 days postpartum were used for non-targeted nuclear magnetic resonance (1H-NMR) analysis to measure the different metabolites of cows with IO. In group B compared with group A at 70 days postpartum, there was an increase in the milk whey of six different metabolites including succinate, creatine phosphate, glycine, myo-inositol, glycolate, and orotate and a decrease in the milk whey of seven metabolites, including alanine, creatinine, o-phosphorylcholine, lactose, taurine, galactose, and glucose-1-phosphate. There was an increase in the serum of group B cows of four differential metabolites, including 3-hydroxybutyrate, acetate, glutamine, and glycine and a decrease in the serum of nine differential metabolites, including alanine, succinate, citrate, creatinine, o-phosphocholine, glucose, myo-inositol, tyrosine, and histidine compared with group A. Group B cows with IO had decreased glucose metabolism and impaired tricarboxylic acid cycle, increased lipid mobilization, and abnormal amino acid metabolism. The study provides a potential prevention strategy for IO in dairy cows in future.
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影响因子：
2.8
作者：
Kuşcu N;Bizzarri M;Bevilacqua A
通讯作者：
Bevilacqua A
影响因子：
3.5
作者：
Duffield, T. F.;Lissemore, K. D.;Leslie, K. E.
通讯作者：
Leslie, K. E.
影响因子：
1.9
作者：
Fair, Trudee
通讯作者：
Fair, Trudee
影响因子：
2.5
作者：
Bell, Alan W.;Bauman, Dale E.
通讯作者：
Bauman, Dale E.
DOI：
10.1016/j.cvfa.2016.01.009
发表时间：
2016-07-01
影响因子：
4.1
作者：
Gilbert, Robert O.
通讯作者：
Gilbert, Robert O.