Depletion of Ly6G/C(+) cells ameliorates delayed cerebral vasospasm in subarachnoid hemorrhage.
Depletion of Ly6G/C(+) cells ameliorates delayed cerebral vasospasm in subarachnoid hemorrhage.
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DOI：
10.1016/j.jneuroim.2010.10.016
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发表时间：
2011-03
影响因子：
3.3
通讯作者：
Ransohoff RM
中科院分区：
文献类型：
--
作者：
Provencio JJ;Altay T;Smithason S;Moore SK;Ransohoff RM
动脉瘤性蛛网膜下腔出血（SAH）后迟发性脑血管痉挛（DCV）的病因尚不清楚。越来越多的证据支持炎症在DCV发病机制中的作用。我们发现脑脊液中性粒细胞可以预测哪些患者会发展成DCV。我们对小鼠SAH模型进行了评估，以验证髓系细胞是DCV相关脑损伤所必需的假设。SAH与第1天大脑中动脉直径减小有关，在第3天恢复正常，第6天复发。此外，巴恩斯迷宫的行为测试显示，在出血后的第7天，执行功能障碍逐渐恶化。为了测试先天免疫反应的作用，我们在实验性SAH之前给药了一种针对Ly6G/C的骨髓细胞消耗单克隆抗体。髓系细胞耗竭改善血管造影血管痉挛测量的MCA血管口径和标准化行为测试。我们的研究结果支持Ly6G/C+细胞在SAH后DCV发展中的作用，并提示中性粒细胞或其他Ly6G/C+细胞的免疫调节可能是预防DCV的一种策略。
The etiology of delayed cerebral vasospasm (DCV) after aneurismal subarachnoid hemorrhage (SAH) has remained elusive. Growing evidence supports a role for inflammation in the pathogenesis of DCV. We showed that CSF neutrophils predict which patients will develop DCV. We evaluated a murine model of SAH to test the hypothesis that myeloid cells are required for the cerebral damage associated with DCV. SAH was associated with decreased middle cerebral artery caliber on day 1 which normalized at day 3 and recurred at day 6. In addition, behavioral testing with a Barnes maze showed executive dysfunction that progressively worsened after the seventh day post hemorrhage. To test the role of innate immune responses, we administrated a myeloid cell-depleting monoclonal antibody against Ly6G/C prior to experimental SAH. Myeloid cell depletion ameliorated angiographic vasospasm measured by MCA vessel caliber and normalized behavioral testing. Our findings support the role of Ly6G/C+ cells in the development of DCV after SAH and suggest that immune modulation of neutrophils or other Ly6G/C+ cells may be a strategy for the prevention of DCV.
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影响因子：
37.8
作者：
Naruko, T;Ueda, M;Becker, AE
通讯作者：
Becker, AE
影响因子：
8.3
作者：
Baldwin, ME;Macdonald, RL;Rosengart, AJ
通讯作者：
Rosengart, AJ
影响因子：
1.9
作者：
Takizawa, T;Tada, T;Uemura, K
通讯作者：
Uemura, K
脑脊液中性粒细胞与蛛网膜下腔出血中血管痉挛的发生有关。
影响因子：
3.5
作者：
Provencio, J. J.;Fu, X.;Siu, A.;Rasmussen, P. A.;Hazen, S. L.;Ransohoff, R. M.
通讯作者：
Ransohoff, R. M.
影响因子：
3
作者：
Altay, Tamer;Smithason, Saksith;Provencio, J. Javier
通讯作者：
Provencio, J. Javier