Preferential induction of c-fos immunoreactivity in vasoactive intestinal polypeptide-innervated gonadotropin-releasing hormone neurons during a steroid-induced luteinizing hormone surge in the female rat.
Preferential induction of c-fos immunoreactivity in vasoactive intestinal polypeptide-innervated gonadotropin-releasing hormone neurons during a steroid-induced luteinizing hormone surge in the female rat.
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在雌性大鼠类固醇诱导的黄体生成素激增期间，优先诱导血管活性肠多肽神经支配的促性腺激素释放激素神经元中的 c-fos 免疫反应性。
作者：
E. van der Beek;H J van Oudheusden;R. Buijs;H A van der Donk;R. van den Hurk;V. M. Wiegant
在小型啮齿类动物中，繁殖严重依赖于大脑昼夜节律振荡器——视交叉上核（SCN）的完整性。 SCN 损伤会导致未绝育雌性大鼠持续发情（不排卵），而切除卵巢的大鼠中植入雌激素会导致每日 LH 激增，而 SCN 损伤后 LH 会消失。血管活性肠多肽 (VIP) 是一种在 SCN 细胞体中合成的肽，与 LH 释放的调节有关。最近，我们为包含 VIP 的神经元从 SCN 投射到 GnRH 系统提供了免疫细胞化学证据。这表明来自 SCN 的 VIP 可能通过直接影响 GnRH 神经元来调节 LH 释放。为了研究 VIP 输入对 GnRH 神经元和 SCN 神经元在 LH 激增产生中的影响，我们使用免疫反应性 c-fos 作为切除卵巢的成熟大鼠和接受类固醇治疗的未成熟大鼠的细胞活化标记物。观察到含有 VIP 的纤维与大部分含有 c-fos 的 GnRH 神经元并置。一般来说，c-fos 的表达在 VIP 神经支配的 GnRH 神经元中比在一般 GnRH 神经元中更常见。这种激活差异在 LH 激增开始时最为明显。在 SCN 神经元中，类固醇治疗在 LH 激增之前或期间不会诱导 c-fos 免疫反应性。目前的结果表明，含 VIP 的纤维可能起源于 SCN，参与了 LH 激增的启动。鉴于已报道的 VIP 对 LH 释放的抑制作用，建议 VIP 输入在这方面的作用是允许的。
In small rodents, reproduction is critically dependent on the integrity of the circadian oscillator of the brain, the suprachiasmatic nucleus (SCN). Lesions of the SCN induce persistent estrus (anovulation) in intact female rats, whereas estrogen implantation in ovariectomized rats results in daily LH surges, which disappear after SCN lesions. Vasoactive intestinal polypeptide (VIP), a peptide synthesized in cell bodies of the SCN, has been implicated in the regulation of LH release. Recently, we have provided immunocytochemical evidence for a VIP-containing neuronal projection from the SCN to the GnRH system. This suggests that VIP from the SCN may modulate LH release via a direct influence on GnRH neurons. To investigate the involvement of VIP input on GnRH neurons and SCN neurons in the generation of a LH surge, we used immunoreactive c-fos as a marker for cell activation in ovariectomized mature rats and immature rats treated with steroids. VIP-containing fibers were observed in apposition to a substantial portion of the GnRH neurons containing c-fos. Expression of c-fos was more frequently observed in VIP-innervated GnRH neurons than in GnRH neurons in general. This difference in activation was most pronounced during the onset of the LH surge. In SCN neurons, steroid treatment did not induce c-fos immunoreactivity before or during the LH surge. The present results indicate that VIP-containing fibers, possibly originating in the SCN, are involved in the initiation of the LH surge. In view of the reported inhibitory effects of VIP on LH release, it is suggested that the role of VIP input in this respect is permissive.
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黄体酮通过增加黄体生成素释放激素神经元的激活来增强黄体生成素的激增。
影响因子：
4.8
作者：
Lee,WS;Smith,MS;Hoffman,GE
通讯作者：
Hoffman,GE
LHRH 神经元在促黄体激素激增期间表达 cJun 蛋白。
影响因子：
4.8
作者：
Lee,WS;Abbud,R;Smith,MS;Hoffman,GE
通讯作者：
Hoffman,GE
影响因子：
56.9
作者：
RALPH, MR;FOSTER, RG;MENAKER, M
通讯作者：
MENAKER, M
大鼠黄体生成素释放激素脉冲发生器的神经内分泌调节。
DOI：
10.1016/b978-0-12-571147-0.50008-1
发表时间：
1991
期刊：
Recent progress in hormone research
影响因子：
--
作者：
Levine,JE;Bauer-Dantoin,AC;Besecke,LM;Conaghan,LA;Legan,SJ;Meredith,JM;Strobl,FJ;Urban,JH;Vogelsong,KM;Wolfe,AM
通讯作者：
Wolfe,AM