¹H-[¹³C]-nuclear magnetic resonance spectroscopy measures of ketamine's effect on amino acid neurotransmitter metabolism.
¹H-[¹³C]-nuclear magnetic resonance spectroscopy measures of ketamine's effect on amino acid neurotransmitter metabolism.
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DOI：
10.1016/j.biopsych.2011.11.006
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发表时间：
2012-06-01
影响因子：
10.6
通讯作者：
Sanacora, Gerard
中科院分区：
文献类型：
--
作者：
Chowdhury, Golam M. I.;Behar, Kevin L.;Cho, William;Thomas, Monique A.;Rothman, Douglas L.;Sanacora, Gerard
关键词：
氯胺酮最近因其拟精神分裂症和最近发现的快速抗抑郁药样特性而受到极大关注。1H-[13C]-核磁共振研究被用来探索这些独特效应背后的潜在生理过程。用[1-13C]葡萄糖和[2-13C]乙酸酯-核磁共振技术对30 mg/kg和80 mg/kg氯胺酮急性染毒大鼠的mPFC和海马神经元进行了体外研究，并与生理盐水组动物进行比较，以确定氯胺酮对氨基酸类神经递质循环和神经胶质代谢的影响。亚麻醉剂量的氯胺酮显著增加大鼠mPFC中谷氨酸、γ-氨基丁酸和谷氨酰胺的~(13)C富集率。亚麻醉剂量的氯胺酮可增加mPFC氨基酸、神经递质循环以及神经元和神经胶质的能量代谢。这些数据补充了之前的报告，表明亚麻醉剂量的氯胺酮后，谷氨酸释放的mPFC水平增加，与药物对认知、知觉和情绪的急性影响有关。
Ketamine has recently gained significant attention owing to its psychotomimetic and more recently discovered rapid antidepressant-like properties. 1H-[13C]-NMR studies were employed to explore potential physiological processes underlying these unique effects. [1-13C]glucose and [2-13C]acetate-NMR ex vivo studies were performed on the mPFC and hippocampus of rats acutely treated with 30mg/kg or 80mg/kg ketamine and compared to saline treated animals to determine the effects of ketamine on amino acid neurotransmitter cycling and glial metabolism. A sub-anesthetic, but not anesthetic, dose of ketamine significantly increased the percentage 13C-enrichments of Glutamate, GABA, and Glutamine in the mPFC of rats. Sub-anesthetic doses of ketamine increase mPFC amino acid neurotransmitter cycling as well as neuronal and glial energy metabolism. These data add to previous reports suggesting increased mPFC levels of glutamate release, following the administration of sub-anesthetic doses of ketamine, are related to the drug’s acute effects on cognition, perception and mood.
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DOI：
10.1126/science.1190287
发表时间：
2010-08-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li N;Lee B;Liu RJ;Banasr M;Dwyer JM;Iwata M;Li XY;Aghajanian G;Duman RS
通讯作者：
Duman RS
影响因子：
2.9
作者：
Duncan, GE;Moy, SS;Breese, GR
通讯作者：
Breese, GR
影响因子：
17.7
作者：
Rowland, LM;Bustillo, JR;Brooks, WM
通讯作者：
Brooks, WM
影响因子：
3.3
作者：
Hackler, E. A.;Byun, N. E.;Gore, J. C.
通讯作者：
Gore, J. C.
影响因子：
2.9
作者：
Iltis, Isabelle;Koski, Dee M.;Henry, Pierre-Gilles
通讯作者：
Henry, Pierre-Gilles