Fundus autofluorescence and the bisretinoids of retina.
Fundus autofluorescence and the bisretinoids of retina.
复制标题
DOI：
10.1039/c0pp00207k
复制
发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Sparrow JR;Wu Y;Nagasaki T;Yoon KD;Yamamoto K;Zhou J
用可见光谱中的激发波长对人眼底成像揭示了天然的自发荧光，其在健康视网膜中主要来源于构成视网膜色素上皮（RPE）细胞的脂褐素的类双视黄酸。由于眼底自发荧光的强度和分布在视网膜疾病的存在下改变，我们已经检查了视网膜bisretinoids的荧光特性，以帮助临床解释。如对于眼底自发荧光也观察到的，来自RPE脂褐质的荧光发射是用宽范围的激发波长产生的;随着激发波长的增加，发射最大值向更长的波长移动，并且光谱宽度减小。这些特征与从化合物的混合物产生荧光一致。虽然构成RPE脂褐质的双维A酸都以中心在600 nm左右的最大值发出荧光，但当在488 nm激发时荧光强度发生变化，488 nm是用于眼底自体荧光成像的激发波长。例如，双视黄酸A2-二氢吡啶-磷脂酰乙醇胺（A2-DHP-PE）的荧光效率大于A2 E，并且相对于后者两者，全反式-视黄酸二聚体-磷脂酰乙醇胺是弱荧光的。另一方面，存在于RPE和感光细胞中的某些光氧化形式的双维甲酸比母体化合物具有更强的荧光。我们还试图评估是否在一些单基因起源的视网膜疾病中观察到的弥漫性自发荧光斑点可归因于类维生素A的积累。然而，两个视黄酸的视觉周期，全反式视黄酯和全反式视网膜，没有表现出荧光在488 nm的激发。
Imaging of the human fundus of the eye with excitation wavelengths in the visible spectrum reveals a natural autofluorescence, that in a healthy retina originates primarily from the bisretinoids that constitute the lipofuscin of retinal pigment epithelial (RPE) cells. Since the intensity and distribution of fundus autofluorescence is altered in the presence of retinal disease, we have examined the fluorescence properties of the retinal bisretinoids with a view to aiding clinical interpretations. As is also observed for fundus autofluorescence, fluorescence emission from RPE lipofuscin was generated with a wide range of exciting wavelengths; with increasing excitation wavelength, the emission maximum shifted towards longer wavelengths and spectral width was decreased. These features are consistent with fluorescence generation from a mixture of compounds. While the bisretinoids that constitute RPE lipofuscin all fluoresced with maxima that were centered around 600 nm, fluorescence intensities varied when excited at 488 nm, the excitation wavelength utilized for fundus autofuorescence imaging. For instance the fluorescence efficiency of the bisretinoid A2-dihydropyridine-phosphatidylethanolamine (A2-DHP-PE) was greater than A2E and relative to both of the latter, all-trans-retinal dimer-phosphatidylethanolamine was weakly fluorescent. On the other hand, certain photooxidized forms of the bisretinoids present in both RPE and photoreceptor cells were more strongly fluorescent than the parent compound. We also sought to evaluate whether diffuse puncta of autofluorescence observed in some retinal disorders of monogenic origin are attributable to retinoid accumulation. However, two retinoids of the visual cycle, all-trans-retinyl ester and all-trans-retinal, did not exhibit fluorescence at 488 nm excitation.
登录
查看更多内容
影响因子：
4.8
作者：
Maeda, Akiko;Maeda, Tadao;Palczewski, Krzysztof
通讯作者：
Palczewski, Krzysztof
影响因子：
13.7
作者：
Lorenz, B;Wabbels, B;Preising, MN
通讯作者：
Preising, MN
DOI：
10.1073/pnas.0708714104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Kim, So R.;Jang, Young P.;Sparrow, Janet R.
通讯作者：
Sparrow, Janet R.
影响因子：
4.4
作者：
Humbert, Ghyslaine;Delettre, Cecile;Hamel, Christian P.
通讯作者：
Hamel, Christian P.
影响因子：
4.4
作者：
Morgan, Jessica I. W.;Hunter, Jennifer J.;Williams, David R.
通讯作者：
Williams, David R.