Anti-inflammatory activity of phlorotannin-rich fermented Ecklonia cava processing by-product extract in lipopolysaccharide-stimulated RAW 264.7 macrophages
Anti-inflammatory activity of phlorotannin-rich fermented Ecklonia cava processing by-product extract in lipopolysaccharide-stimulated RAW 264.7 macrophages
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富含叶皮单宁的发酵昆布茶加工副产物提取物在脂多糖刺激的 RAW 264.7 巨噬细胞中的抗炎活性
DOI：
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发表时间：
2013
影响因子：
3.3
通讯作者：
Y. Jeon
中科院分区：
文献类型：
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作者：
W. Wijesinghe;G. Ahn;Wonwoo Lee;Min;Eun;Y. Jeon
在本研究中，利用发酵作为进一步提高从食品和化妆品工业中提取多酚后的褐海藻加工副产物的生物活性潜力的工具。发酵过程使用一种具有重要工业意义的微生物念珠菌进行24小时，然后用80%的乙醇提取。采用体外抗炎实验研究了肠杆菌发酵副产物提取物(FEPBE)的抗炎作用。富含邻苯二甲酸的FEPBE可剂量依赖性地抑制脂多糖刺激的RAW 264.7细胞一氧化氮和前列腺素E_2的产生，并抑制诱导型一氧化氮合酶和环氧合酶-2的表达。对促炎症细胞因子IL-1β和IL-6的释放有明显抑制作用，且呈剂量依赖关系。由于具有深刻的抗炎活性，FEPBE是一种高附加值的生物质组分，可作为功能性成分的来源在众多工业应用中开发利用。
In this study, fermentation was employed as a tool to further increase the bioactive potential of processing by-product from a brown seaweed, Ecklonia cava, which can be obtained from food and cosmetic industries after its polyphenol extraction. The fermentation process was done for 24 h using an industrially important microorganism Candida utilis prior to being extracted with 80 % ethanol. The anti-inflammatory potential of the fermented E. cava processing by-product extract (FEPBE) was evaluated in vitro. The phlorotannin-rich FEPBE dose-dependently inhibited the nitric oxide production, prostaglandin-E2 production and suppressed the inducible nitric oxide synthase and cyclooxygenase-2 expressions in lipopolysaccharide-stimulated RAW 264.7 cells. The release of pro-inflammatory cytokines, interleukin-1β and interleukin-6, was significantly suppressed by the extract in a dose-dependent manner. Due to the profound anti-inflammatory activity, FEPBE appears as a value-added biomass fraction that can be exploited in numerous industrial applications as a source of functional ingredients.
影响因子：
4.5
作者：
Kassim, Mustafa;Achoui, Mouna;Yusoff, Kamaruddin Mohd
通讯作者：
Yusoff, Kamaruddin Mohd