Global Investigation of TBL Gene Family in Rose (Rosa chinensis) Unveils RcTBL16 Is a Susceptibility Gene in Gray Mold Resistance.
Global Investigation of TBL Gene Family in Rose (Rosa chinensis) Unveils RcTBL16 Is a Susceptibility Gene in Gray Mold Resistance.
复制标题
对玫瑰（Rosa chinensis）TBL基因家族的全球调查揭示了RcTBL16是灰霉病抗性的易感基因
DOI：
10.3389/fpls.2021.738880
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Tian Y;Zhang S;Liu X;Zhang Z
TRICHOME BIFRINGENCE-LIKE（TBL）家族是参与细胞壁多糖O-乙酰化的重要基因家族。已有报道表明TBL参与了拟南芥和水稻对植物病原菌的抗性。然而，没有相关的研究玫瑰（Rosa sp.）已出版。本研究对月季TBL基因家族进行了全基因组分析，包括其系统发育关系、基因结构、染色体定位和共线性分析。系统发育分析显示，玫瑰基因组中共有50个RcTBL基因，并且它们在所有7条染色体上分布不均匀。基因重复事件的发生表明，全基因组重复和部分重复都可能在RcTBL的基因重复中起作用。Ka/Ks分析表明，复制的RcTBL基因主要经过纯化选择，功能分化有限。基因表达分析表明，12个RcTBL在灰霉病菌感染后表达下调。这些RcTBL可能是调控玫瑰对B反应的候选基因。灰叶通过病毒诱导的基因沉默，RcTBL 16被证明与玫瑰对灰霉病的易感性相关。本研究为进一步研究月季TBL蛋白家族的功能提供了有意义的信息。
The TRICHOME BIREFRINGENCE-LIKE (TBL) family is an important gene family engaged in the O-acetylation of cell wall polysaccharides. There have been a few reports showing that TBL participated in the resistance against phytopathogens in Arabidopsis and rice. However, no relevant studies in rose (Rosa sp.) have been published. In this study, a genome-wide analysis of the TBL gene family in rose was presented, including their phylogenetic relationships, gene structure, chromosomal positioning, and collinearity analysis. The phylogenetic analysis revealed a total of 50 RcTBL genes in the rose genome, and they are unevenly distributed across all seven chromosomes. The occurrence of gene duplication events suggests that both the whole genome duplication and partial duplication may play a role in gene duplication of RcTBLs. The analysis of Ka/Ks showed that the replicated RcTBL genes underwent mainly purifying selection with limited functional differentiation. Gene expression analysis indicated that 12 RcTBLs were down-regulated upon the infection of Botrytis cinerea, the causal agent of the gray mold disease of rose. These RcTBLs may be a sort of candidate genes for regulating the response of rose to B. cinerea. Through virus-induced gene silencing, RcTBL16 was shown to be associated with susceptibility to gray mold in rose. Through this study, meaningful information for further studies on the function of the TBL protein family in rose is provided.
登录
查看更多内容
分离花瓣盘测定和病毒诱导的基因沉默促进了月季花灰霉病抗性的研究
影响因子：
8.7
作者：
Cao, Xiaoqian;Yan, Huijun;Zhang, Zhao
通讯作者：
Zhang, Zhao
影响因子：
7.4
作者：
Manabe, Yuzuki;Nafisi, Majse;Scheller, Henrik Vibe
通讯作者：
Scheller, Henrik Vibe
影响因子：
7.2
作者：
Vogel, JP;Raab, TK;Somerville, SC
通讯作者：
Somerville, SC
影响因子：
7.4
作者：
Manabe, Yuzuki;Verhertbruggen, Yves;Scheller, Henrik Vibe
通讯作者：
Scheller, Henrik Vibe
影响因子：
4.9
作者：
Zhong, Ruiqin;Cui, Dongtao;Ye, Zheng-Hua
通讯作者：
Ye, Zheng-Hua