Crystals of Escherichia coli single-strand DNA-binding protein show that the tetramer has D2 symmetry.
Crystals of Escherichia coli single-strand DNA-binding protein show that the tetramer has D2 symmetry.
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大肠杆菌单链DNA结合蛋白晶体显示四聚体具有D2对称性。
DOI:
10.1016/s0022-2836(83)80134-x
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发表时间:
1983
影响因子:
5.6
通讯作者:
Steitz,TA
中科院分区:
文献类型:
--
作者:
Ollis,D;Brick,P;Abdel-Meguid,SS;Murthy,K;Chase,JW;Steitz,TA
Crystals ofEscherichia colisingle-strand DNA-binding protein (SSB) that are suitable for high-resolution. X-ray crystallographic studies have been obtained. Form I crystals are monoclinic space groupC2 with unit cell dimensions ofa= 105·5 Å,b= 62·7 Å,c= 98·1 Å, β = 112·6°, and with an asymmetric unit containing two native and two proteolytically degraded SSB subunits. Form II crystals are hexagonal space groupP62(4)22 witha=b= 61·4 Å andc= 264·3 Å and with two chymotryptic fragment SSB subunits per asymmetric unit. Calculation of rotation function using 6 Å data shows that SSB hasD2symmetry.
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