Crystals of Escherichia coli single-strand DNA-binding protein show that the tetramer has D2 symmetry.

Crystals of Escherichia coli single-strand DNA-binding protein show that the tetramer has D2 symmetry.
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大肠杆菌单链DNA结合蛋白晶体显示四聚体具有D2对称性。

DOI:
10.1016/s0022-2836(83)80134-x
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发表时间:
1983
影响因子:
5.6
通讯作者:
Steitz,TA
Steitz,TA
中科院分区:
生物学2区
文献类型:
--
作者:
Ollis,D;Brick,P;Abdel-Meguid,SS;Murthy,K;Chase,JW;Steitz,TA

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适用于高分辨率的大肠埃希氏菌DNA结合蛋白(SSB)晶体。获得了X射线结晶学研究结果。I型晶体为单斜晶系C2,晶胞尺寸为a=105.5o,b=62.7o,c=98.1o,β=112.6°,不对称晶胞中含有两个天然亚基和两个蛋白降解亚基。第二类晶体为六方空间群P62(4)22,a=b=61·4?,c=264·3?,每个不对称单位有两个凝乳酶片段SSB亚基。用6?数据计算的旋转函数表明,单边带具有D2对称性。
Crystals ofEscherichia colisingle-strand DNA-binding protein (SSB) that are suitable for high-resolution. X-ray crystallographic studies have been obtained. Form I crystals are monoclinic space groupC2 with unit cell dimensions ofa= 105·5 Å,b= 62·7 Å,c= 98·1 Å, β = 112·6°, and with an asymmetric unit containing two native and two proteolytically degraded SSB subunits. Form II crystals are hexagonal space groupP62(4)22 witha=b= 61·4 Å andc= 264·3 Å and with two chymotryptic fragment SSB subunits per asymmetric unit. Calculation of rotation function using 6 Å data shows that SSB hasD2symmetry.
DOI: --
发表时间: 1979
影响因子: 5.6
作者:
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通讯作者: A. Rich
大肠杆菌中单链 DNA 结合蛋白的扩增。
DOI: 10.1093/nar/8.14.3215
发表时间: 1980
影响因子: 14.9
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DOI: 10.1016/0022-2836(82)90321-7
发表时间: 1982
影响因子: 5.6
作者:
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通讯作者: Williams,KR
DOI: 10.1021/bi00668a017
发表时间: 1976-01-01
期刊: BIOCHEMISTRY
影响因子: 2.9
作者:
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