Short and long term, in vitro and in vivo correlations of cellular and tissue responses to mesoporous silicon nanovectors.
Short and long term, in vitro and in vivo correlations of cellular and tissue responses to mesoporous silicon nanovectors.
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DOI：
10.1002/smll.201201939
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发表时间：
2013-05-27
期刊：
影响因子：
13.3
通讯作者：
Tasciotti, Ennio
中科院分区：
文献类型：
--
作者：
Martinez, Jonathan O.;Boada, Christian;Yazdi, Iman K.;Evangelopoulos, Michael;Brown, Brandon S.;Liu, Xuewu;Ferrari, Mauro;Tasciotti, Ennio
纳米材料的特性及其对细胞和组织生物学的影响和相互作用仍然部分未知。基于中孔硅的多级纳米载体已被广泛研究用于药物输送、热加热和改善诊断成像。在这里，我们分析了介孔硅纳米载体（MSV）内化后人类细胞中发生的短期和长期变化。使用定性和定量技术以及体外和体内生化，细胞和功能测定，我们证明了MSV没有引起任何显着的急性或慢性影响的细胞和组织。我们分析了体外细胞毒性和活力，细胞周期的维持，以及MSV内化后的结构。此外，我们评估了MSV是否产生任何促炎反应，并研究了其体内生物相容性。我们使用纵向体内成像跟踪MSV的生物分布，并使用定量元素和荧光技术评估器官蓄积。最后，对采集的组织进行的全面病理学分析表明，肝脏中存在轻度一过性全身反应，该反应在颗粒清除后消失。总之，通过这项研究，我们提出，未来的努力，旨在了解裸露的药物载体的毒理学，应旨在解决其影响，在体外和体内的短期和长期的全身反应评价。
The characterization of nanomaterials and their influence and interactions on the biology of cells and tissues are still partially unknown. Multistage nanovectors based on mesoporous silicon have been extensively studied for drug delivery, thermal heating and improved diagnostic imaging. Here we analyzed the short and long-term changes occurring in human cells upon the internalization of mesoporous silicon nanovectors (MSV). Using qualitative and quantitative techniques as well as in vitro and in vivo biochemical, cellular and functional assays, we demonstrated that MSV did not cause any significant acute or chronic effects on cells and tissues. We analyzed in vitro cell toxicity and viability, the maintenance of cell phase cycling, and the architecture upon the internalization of MSV. In addition, we evaluated if MSV produced any pro-inflammatory response and studied its biocompatibility in vivo. We followed the biodistribution of MSV using longitudinal in vivo imaging and assessed organ accumulation using quantitative elemental and fluorescent techniques. Finally, a thorough pathological analysis of collected tissues demonstrated a mild transient systemic response in the liver that dissipated upon clearance of particles. In conclusion, with this study we propose that future endeavors aimed at understanding the toxicology of naked drug carriers should be designed to address their impact using in vitro and in vivo short and long-term evaluations of systemic response.
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影响因子：
14
作者：
Decuzzi, Paolo;Ferrari, Mauro
通讯作者：
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影响因子：
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