Type I alveolar epithelial phenotype in primary culture.
Type I alveolar epithelial phenotype in primary culture.
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原代培养物中的 I 型肺泡上皮表型。
DOI：
10.1165/rcmb.2009-0359oc
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发表时间：
2011
影响因子：
6.4
通讯作者：
Hubmayr,RolfD
中科院分区：
文献类型：
--
作者：
Wang,Shaohua;Hubmayr,RolfD
Type I alveolar epithelial cells (ATIs) are very large, thin cells, which extend across several air sacs and cover more than 95% of the alveolar surface area. ATIs are the target of many insults, including ventilator-induced lung injury, and are generally considered terminally differentiated cells arising from type II cell (ATII) lineage. ATIs have proven difficult to harvest and maintain in primary culture, which is why much of ATI biology has been inferred from studies onex vivo, ATII-derived, so-called ATI-like cells. We report on a modified approach to rat ATI harvest and primary culture, which yielded the following observations: (1) rat ATI can be harvested and maintained with a high degree of purity in primary culture; (2)in vitrogrowth characteristics of primary ATIs differ from those of ATII-derived ATI-like cells; ATIs, but notex vivo, ATII-derived ATI-like cells, are capable of cell division; (3) ATIs readily repair plasma membrane wounds without the subsequent loss of their ability to divide; (4) ATI monolayers heal scratch wounds primarily by cell spreading and migration. Although the ability of ATIs to divide may be limited to thein vitroenvironment, we do believe that their role in alveolar wound repair deserves to be revisited, and the molecular control of ATI–ATII plasticity further explored.
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DOI：
10.1073/pnas.95.6.2991
发表时间：
1998-03-17
影响因子：
11.1
作者：
Dobbs, LG;Gonzalez, R;Verkman, AS
通讯作者：
Verkman, AS
呼吸组织：结构、组织生理学、细胞动力学。
DOI：
10.1016/s0074-7696(08)60408-8
发表时间：
1964
期刊：
International review of cytology
影响因子：
--
作者：
F. Bertalanffy
通讯作者：
F. Bertalanffy
影响因子：
15.9
作者：
PANOS, RJ;RUBIN, JS;MASON, RJ
通讯作者：
MASON, RJ
DOI：
10.1165/rcmb.2005-0175rc
发表时间：
2005-10-01
影响因子：
6.4
作者：
Kotton, DN;Fabian, AJ;Mulligan, RC
通讯作者：
Mulligan, RC
DOI：
10.1016/s0232-1513(82)80001-7
发表时间：
1982-01-01
期刊：
EXPERIMENTAL PATHOLOGY
影响因子：
--
作者：
ANISIMOV, VN
通讯作者：
ANISIMOV, VN