Characterization of Strip1 Expression in Mouse Cochlear Hair Cells.
Characterization of Strip1 Expression in Mouse Cochlear Hair Cells.
复制标题
小鼠耳蜗毛细胞中 Strip1 表达的表征
DOI：
10.3389/fgene.2021.625867
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发表时间：
2021
影响因子：
3.7
通讯作者：
Chai R
中科院分区：
文献类型：
--
作者：
Zhang S;Dong Y;Qiang R;Zhang Y;Zhang X;Chen Y;Jiang P;Ma X;Wu L;Ai J;Gao X;Wang P;Chen J;Chai R
纹状体相互作用蛋白1（Strip 1）是纹状体相互作用磷酸酶和激酶（STRIPAK）复合物的核心组分，其参与胚胎发生和发育、昼夜节律、2型糖尿病和癌症进展。然而，Strip 1在哺乳动物耳蜗中的表达和作用仍不清楚。本研究利用Strip 1基因敲除小鼠研究Strip 1在小鼠耳蜗中的表达和功能。我们首先发现Strip 1的mRNA和蛋白表达从出生后第3天开始随着小鼠年龄的增长而增加，并在P30达到最高表达水平，并且Strip 1的表达可以通过免疫荧光染色从P14开始仅在耳蜗毛细胞中检测到，而不在支持细胞中检测到。接下来，我们将Strip 1杂合敲除（Strip 1 +/−）小鼠杂交，获得Strip 1纯合敲除（Strip 1 −/−）小鼠，用于研究Strip 1在耳蜗毛细胞中的作用。然而，没有获得Strip 1 −/−小鼠，每窝Strip 1 +/−与Strip 1 +/+小鼠的比例约为2：1，这表明纯合子Strip 1敲除是胚胎致死的。我们测量了P30和P60 Strip +/−小鼠的听力功能并计算了HC数量，发现与Strip 1 +/+小鼠相比，它们具有正常的听力能力和HC数量。我们的研究提示Strip 1可能在HC的发育和成熟过程中发挥重要作用，这一点有待于进一步研究。
Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.
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