Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria.
Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria.
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DOI：
10.1099/mic.0.2007/008904-0
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发表时间：
2007-08
期刊：
影响因子：
--
通讯作者：
Hatfull GF
中科院分区：
文献类型：
--
作者：
Pham TT;Jacobs-Sera D;Pedulla ML;Hendrix RW;Hatfull GF
分枝杆菌噬菌体Tweety是一种新分离的耻垢分枝杆菌噬菌体。其病毒形态具有等长的头部和长而灵活的尾部，并形成混浊的噬斑，从中可以分离出稳定的溶原。Tweety基因组长度为58 692 bp，包含109个蛋白质编码基因，并显示出与先前描述的分枝杆菌噬菌体Llij，PMC和Che 8显着但中断的核苷酸序列相似性。 然而，总的来说，基因组具有镶嵌结构，基因产物与其他分枝杆菌噬菌体如Che 9d，Omega和Corndog相关。编码酪氨酸重组酶家族的整合酶的基因位于基因组的中心附近，并且在int上游的一个短的基因间区域内已经鉴定了一个推定的attP位点。该Tweety attP-int盒用于构建一组新的整合熟练的质粒载体，其有效地转化快-和慢-通过质粒整合在含有tRNALys基因的染色体基因座上生长分枝杆菌。这些载体在没有选择的情况下保持良好，并且与来自分枝杆菌噬菌体L5的整合载体完全相容，使得能够简单地构建具有同时整合在不同染色体位置的基因的复杂重组体。
Mycobacteriophage Tweety is a newly isolated phage of Mycobacterium smegmatis. It has a viral morphology with an isometric head and a long flexible tail, and forms turbid plaques from which stable lysogens can be isolated. The Tweety genome is 58 692 bp in length, contains 109 protein-coding genes, and shows significant but interrupted nucleotide sequence similarity with the previously described mycobacteriophages Llij, PMC and Che8. However, overall the genome possesses mosaic architecture, with gene products being related to other mycobacteriophages such as Che9d, Omega and Corndog. A gene encoding an integrase of the tyrosine-recombinase family is located close to the centre of the genome, and a putative attP site has been identified within a short intergenic region immediately upstream of int. This Tweety attP–int cassette was used to construct a new set of integration-proficient plasmid vectors that efficiently transform both fast- and slow-growing mycobacteria through plasmid integration at a chromosomal locus containing a tRNALys gene. These vectors are maintained well in the absence of selection and are completely compatible with integration vectors derived from mycobacteriophage L5, enabling the simple construction of complex recombinants with genes integrated simultaneously at different chromosomal positions.
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影响因子：
2.8
作者：
Freitas-Vieira, A;Anes, E;Moniz-Pereira, J
通讯作者：
Moniz-Pereira, J
影响因子：
14.9
作者：
Lewis, JA;Hatfull, GF
通讯作者：
Hatfull, GF
影响因子：
--
作者：
Vultos TD;Méderlé I;Abadie V;Pimentel M;Moniz-Pereira J;Gicquel B;Reyrat JM;Winter N
通讯作者：
Winter N
影响因子：
64.5
作者：
Ojha, A;Anand, M;Hatfull, GF
通讯作者：
Hatfull, GF
影响因子：
3.2
作者：
Parish, T;Lewis, J;Stoker, NG
通讯作者：
Stoker, NG