SlTPR1, a tomato tetratricopeptide repeat protein, interacts with the ethylene receptors NR and LeETR1, modulating ethylene and auxin responses and development.
SlTPR1, a tomato tetratricopeptide repeat protein, interacts with the ethylene receptors NR and LeETR1, modulating ethylene and auxin responses and development.
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DOI：
10.1093/jxb/ern276
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发表时间：
2008
影响因子：
6.9
通讯作者：
Grierson D
中科院分区：
文献类型：
--
作者：
Lin Z;Arciga-Reyes L;Zhong S;Alexander L;Hackett R;Wilson I;Grierson D
气体激素乙烯由与Raf-like激酶CTR 1相互作用的乙烯受体家族感知。SlTPR 1编码一种新的TPR（tetratricopeptide repeat）蛋白，其来自番茄，在酵母双杂交和体外蛋白质相互作用测定中与乙烯受体NR和LeETR 1相互作用。在拟南芥中，带有GFP荧光标记的SlTPR 1蛋白定位于质膜和核膜中，并且在洋葱细胞的共轰击后，SlTPR 1-CFP和NR-YFP融合蛋白共定位于质膜和核膜中。SlTPR 1在番茄中的过表达导致乙烯相关的多效性效应，包括降低身高，延迟和减少花序的产生，异常和不育的花退化的风格和花粉，上位性，减少顶端优势，抑制脱落，改变叶形态，单性结实的果实。在过表达SlTPR 1的拟南芥中观察到类似的表型。SlTPR 1过表达并不增加乙烯的产生，但引起来自乙烯响应基因ChitB和生长素响应基因SlSAUR 1样的mRNA的积累增强，并降低生长素早期响应基因LeIAA 9的表达，已知该基因被乙烯抑制并与单性结实相关。从SlTPR 1-overexpressors扦插产生较少的不定根，吲哚丁酸的反应较低。这表明，SlTPR 1过表达增强了一个子集的乙烯和生长素的反应，通过与特定的乙烯受体相互作用。SlTPR 1与人TTC 1具有共同的特征，其与异源三聚体G蛋白和Ras相互作用，并与Raf-1竞争Ras结合。提出了涉及乙烯信号传导或受体水平调节的SlTPR 1作用模型。
The gaseous hormone ethylene is perceived by a family of ethylene receptors which interact with the Raf-like kinase CTR1. SlTPR1 encodes a novel TPR (tetratricopeptide repeat) protein from tomato that interacts with the ethylene receptors NR and LeETR1 in yeast two-hybrid and in vitro protein interaction assays. SlTPR1 protein with a GFP fluorescent tag was localized in the plasmalemma and nuclear membrane in Arabidopsis, and SlTPR1-CFP and NR-YFP fusion proteins were co-localized in the plasmalemma and nuclear membrane following co-bombardment of onion cells. Overexpression of SlTPR1 in tomato resulted in ethylene-related pleiotropic effects including reduced stature, delayed and reduced production of inflorescences, abnormal and infertile flowers with degenerate styles and pollen, epinasty, reduced apical dominance, inhibition of abscission, altered leaf morphology, and parthenocarpic fruit. Similar phenotypes were seen in Arabidopsis overexpressing SlTPR1. SlTPR1 overexpression did not increase ethylene production but caused enhanced accumulation of mRNA from the ethylene responsive gene ChitB and the auxin-responsive gene SlSAUR1-like, and reduced expression of the auxin early responsive gene LeIAA9, which is known to be inhibited by ethylene and to be associated with parthenocarpy. Cuttings from the SlTPR1-overexpressors produced fewer adventitious roots and were less responsive to indole butyric acid. It is suggested that SlTPR1 overexpression enhances a subset of ethylene and auxin responses by interacting with specific ethylene receptors. SlTPR1 shares features with human TTC1, which interacts with heterotrimeric G-proteins and Ras, and competes with Raf-1 for Ras binding. Models for SlTPR1 action are proposed involving modulation of ethylene signalling or receptor levels.
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