The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves.
The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves.
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两种转录因子 ABS5/bHLH30 和 ABS7/MYB101 的过度表达导致叶子向上卷曲
DOI：
10.1371/journal.pone.0107637
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yu F
中科院分区：
文献类型：
--
作者：
An R;Liu X;Wang R;Wu H;Liang S;Shao J;Qi Y;An L;Yu F
正常的叶片发育是植物生长发育的基础，叶片的形态形成受到复杂的遗传和环境因素网络的控制。我们有兴趣从遗传学上解剖这些调控回路，并在这里报告了两种拟南芥显性突变体的分离，异常射击5- 1d （abs5-1D）和abs7-1D通过激活标记筛选鉴定。abs5-1D和abs7-1D都显示出有趣的向上卷曲的叶片表型。分子克隆结果表明，bHLH转录因子ABS5/T5L1/bHLH30或MYB转录因子ABS7/MYB101的表达升高是导致ABS5 - 1d和ABS7 - 1d叶片表型异常的原因。原生质体瞬时表达实验证实，ABS5/T5L1和ABS7/MYB101均靶向细胞核。有趣的是，生长素反应报告基因DR5::GUS在ABS5 - 1d和ABS5/T5L1过表达系的叶片中表达域异常。此外，子叶脉序分析表明，ab5 - 1d有更多的微孔和自由端脉形成。我们发现，拟南芥分生组织1层启动子（PAtML1）驱动的ABS5/T5L1或ABS7/MYB101的表皮特异性表达足以再现ABS5 - 1d或ABS7 - 1d的卷曲叶表型。此外，PAtML1::ABS5细胞系DR5::GUS表达模式的变化与35s驱动的ABS5/T5L1过表达细胞系相似。我们的研究表明，两种转录因子ABS5/T5L1和ABS7/MYB101的表达增强能够改变叶片的发育，并加强了叶表皮在调节植物器官形态发生中起关键作用的观点。
Proper leaf development is essential for plant growth and development, and leaf morphogenesis is under the control of intricate networks of genetic and environmental cues. We are interested in dissecting these regulatory circuits genetically and report here the isolation of two Arabidopsis dominant mutants, abnormal shoot5-1D (abs5-1D) and abs7-1D identified through activation tagging screens. Both abs5-1D and abs7-1D display an intriguing upwardly curly leaf phenotype. Molecular cloning showed that the elevated expression of a bHLH transcription factor ABS5/T5L1/bHLH30 or a MYB transcription factor ABS7/MYB101 is the cause for the abnormal leaf phenotypes found in abs5-1D or abs7-1D, respectively. Protoplast transient expression assays confirmed that both ABS5/T5L1 and ABS7/MYB101 are targeted to the nucleus. Interestingly, the expression domains of auxin response reporter DR5::GUS were abnormal in leaves of abs5-1D and ABS5/T5L1 over-expression lines. Moreover, cotyledon venation analysis showed that more areoles and free-ending veins are formed in abs5-1D. We found that the epidermis-specific expressions of ABS5/T5L1 or ABS7/MYB101 driven by the Arabidopsis Meristem Layer 1 promoter (PAtML1) were sufficient to recapitulate the curly leaf phenotype of abs5-1D or abs7-1D. In addition, PAtML1::ABS5 lines exhibited similar changes in DR5::GUS expression patterns as those found in 35S-driven ABS5/T5L1 over-expression lines. Our work demonstrated that enhanced expressions of two transcription factors, ABS5/T5L1 and ABS7/MYB101, are able to alter leaf lamina development and reinforce the notion that leaf epidermis plays critical roles in regulating plant organ morphogenesis.
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