Abelson phosphorylation of CLASP2 modulates its association with microtubules and actin.
Abelson phosphorylation of CLASP2 modulates its association with microtubules and actin.
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CLASP2 的 Abelson 磷酸化调节其与微管和肌动蛋白的关联。
DOI：
10.1002/cm.21164
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发表时间：
2014-03
期刊：
影响因子：
2.9
通讯作者：
VanVactor, David
中科院分区：
文献类型：
--
作者：
Engel, Ulrike;Zhan, Yougen;Long, Jennifer B.;Boyle, Scott N.;Ballif, Bryan A.;Dorey, Karel;Gygi, Steven P.;Koleske, Anthony J.;VanVactor, David
Abelson（Abl）非受体酪氨酸激酶在神经发育的多个阶段调节细胞骨架，从神经形成到轴突和树突的连接，再到突触的形成和维持。我们以前表明，Abl是遗传连锁的微管（MT）加上端跟踪蛋白（+TIP）CLASP在果蝇。在这里，我们显示在脊椎动物细胞中，Abl结合CLASP并磷酸化它以响应血清或PDGF刺激。在体外，Abl以1.89 µM的Km磷酸化CLASP，表明CLASP是真正的底物。我们通过质谱法在CLASP中检测到的α-磷酸化酪氨酸残基位于先前绘制的F-肌动蛋白和MT加末端相互作用结构域内。使用纯化的蛋白质，我们发现，Abl磷酸化调节纯化的CLASP 2与MT和肌动蛋白之间的直接结合。与这些观察结果相一致的是，CLASP 2的β-诱导的磷酸化调节其定位以及脊髓生长锥中F-肌动蛋白结构的分布。我们的数据表明，Abl和CLASP 2之间的功能关系是保守的，并提供了一种手段来控制CLASP 2协会与细胞骨架。© 2014作者。出版社：Wiley Periodicals，Inc.
The Abelson (Abl) non-receptor tyrosine kinase regulates the cytoskeleton during multiple stages of neural development, from neurulation, to the articulation of axons and dendrites, to synapse formation and maintenance. We previously showed that Abl is genetically linked to the microtubule (MT) plus end tracking protein (+TIP) CLASP in Drosophila. Here we show in vertebrate cells that Abl binds to CLASP and phosphorylates it in response to serum or PDGF stimulation. In vitro, Abl phosphorylates CLASP with a Km of 1.89 µM, indicating that CLASP is a bona fide substrate. Abl-phosphorylated tyrosine residues that we detect in CLASP by mass spectrometry lie within previously mapped F-actin and MT plus end interaction domains. Using purified proteins, we find that Abl phosphorylation modulates direct binding between purified CLASP2 with both MTs and actin. Consistent with these observations, Abl-induced phosphorylation of CLASP2 modulates its localization as well as the distribution of F-actin structures in spinal cord growth cones. Our data suggest that the functional relationship between Abl and CLASP2 is conserved and provides a means to control the CLASP2 association with the cytoskeleton. © 2014 The Authors. Cytoskeleton Published by Wiley Periodicals, Inc.
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影响因子：
7.8
作者：
Komarova, Yulia A;Akhmanova, Anna S;Kojima, Shin-Ichiro;Galjart, Niels;Borisy, Gary G
通讯作者：
Borisy, Gary G
影响因子：
7.3
作者：
Colicelli J
通讯作者：
Colicelli J
影响因子：
64.5
作者：
Akhmanova, A;Hoogenraad, CC;Galjart, N
通讯作者：
Galjart, N
影响因子：
10.5
作者：
GERTLER, FB;COMER, AR;HOFFMANN, FM
通讯作者：
HOFFMANN, FM
影响因子：
10.5
作者：
Hur, Eun-Mi;Saijilafu;Zhou, Feng-Quan
通讯作者：
Zhou, Feng-Quan