Plasmodium falciparum infection significantly impairs placental cytokine profile in HIV infected Cameroonian women.
Plasmodium falciparum infection significantly impairs placental cytokine profile in HIV infected Cameroonian women.
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DOI：
10.1371/journal.pone.0008114
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发表时间：
2009-12-02
期刊：
影响因子：
3.7
通讯作者：
ANRS 1267 study team
中科院分区：
文献类型：
--
作者：
Kfutwah A;Mary JY;Lemen B;Leke R;Rousset D;Barré-Sinoussi F;Nerrienet E;Menu E;Ayouba A;ANRS 1267 study team
胎盘细胞因子在妊娠的建立和维持以及保护胎儿免受感染中起着至关重要的作用。先前的研究表明，感染如恶性疟原虫和艾滋病毒在胎盘细胞因子的刺激的含义。本研究评估了恶性疟原虫对HIV-1阳性和阴性妇女胎盘细胞因子谱的影响。用厚血涂片检查HIV-1阴性和阳性妇女外周血和胎盘血中恶性疟原虫感染情况。细胞因子蛋白和信使rna分别用ELISA和real - time PCR进行定量。采用非参数检验进行统计分析。胎盘和外周血恶性疟原虫感染与HIV-1感染无显著相关性(OR: 1.4； 95%可信区间（95% ci）： 0.5-4.2；p = 0.50, OR: 0.6；95%置信区间:0.3—-1.4;P = 0.26)。相反，胎盘恶性疟原虫血症在HIV-1阳性组中显著升高（p = 0.04）。我们观察到，与HIV阴性妇女相比，HIV-1阳性妇女胎盘中TNF-α mRNA中位数水平升高（p = 0.02）， IL-10 mRNA有下降趋势（p = 0.07），导致HIV-1阳性妇女胎盘中位数TNF-α/IL-10 mRNA比值显著高于HIV-1阴性妇女胎盘（p = 0.004； 1.5和0.8）。在HIV-1阳性妇女的胎盘中，与HIV阴性妇女相比，分泌细胞因子中位数水平显著下降，但这些结果在一定程度上具有指示性，因为HIV-1阳性妇女和阴性妇女之间细胞因子水平（蛋白质或mRNA）的差异在很大程度上取决于恶性疟原虫感染。在HIV-1阳性组中，TNF-α是唯一与HIV-1 MTCT相关的临床参数（如膜早破、CD4 t细胞数量、血浆病毒载量和分娩前NVP摄入延迟）显著相关的细胞因子。这些结果表明，恶性疟原虫感染深刻地改变了胎盘细胞因子环境，并作为一个混杂因素，掩盖了HIV-1对合并感染妇女的影响。这两种感染之间的相互作用可能对HIV-1和恶性疟原虫共同传播地区的子宫内HIV-1 MTCT有影响。
Placental cytokines play crucial roles in the establishment and maintenance of pregnancy as well as protecting the foetus from infections. Previous studies have suggested the implication of infections such as P. falciparum and HIV in the stimulation of placental cytokines. This study assessed the impact of P. falciparum on placental cytokine profiles between HIV-1 positive and negative women. P. falciparum infection was checked in peripheral and placental blood of HIV-1 negative and positive women by the thick blood smear test. Cytokines proteins and messenger RNAs were quantified by ELISA and real time PCR, respectively. Non-parametric tests were used for statistical analyses. Placental and peripheral P. falciparum infections were not significantly associated with HIV-1 infection (OR: 1.4; 95% confidence interval (95%CI): 0.5–4.2; p = 0.50 and OR: 0.6; 95%CI: 0.3–1.4; p = 0.26, respectively). Conversely, placental P. falciparum parasitemia was significantly higher in the HIV-1 positive group (p = 0.04). We observed an increase of TNF-α mRNA median levels (p = 0.02) and a trend towards a decrease of IL-10 mRNA (p = 0.07) in placenta from HIV-1 positive women compared to the HIV negative ones leading to a median TNF-α/IL-10 mRNA ratio significantly higher among HIV-1 positive than among HIV-1 negative placenta (p = 0.004; 1.5 and 0.8, respectively). Significant decrease in median secreted cytokine levels were observed in placenta from HIV-1 positive women as compared to the HIV negative however these results are somewhat indicative since it appears that differences in cytokine levels (protein or mRNA) between HIV-1 positive and negative women depend greatly on P.falciparum infection. Within the HIV-1 positive group, TNF-α was the only cytokine significantly associated with clinical parameters linked with HIV-1 MTCT such as premature rupture of membranes, CD4 T-cell number, plasma viral load and delay of NVP intake before delivery. These results show that P. falciparum infection profoundly modifies the placenta cytokine environment and acts as a confounding factor, masking the impact of HIV-1 in co-infected women. This interplay between the two infections might have implications in the in utero MTCT of HIV-1 in areas where HIV-1 and P. falciparum co-circulate.
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DOI：
10.1097/00126334-200311010-00003
发表时间：
2003-11-01
影响因子：
3.6
作者：
Ayouba, A;Tene, G;Nerrienet, E
通讯作者：
Nerrienet, E
影响因子：
56.9
作者：
Fried, M;Duffy, PE
通讯作者：
Duffy, PE
影响因子：
3.3
作者：
Kfutwah, Anfumbom K. W.;Mary, Jean-Yves;Menu, Elisabeth
通讯作者：
Menu, Elisabeth
影响因子：
6.4
作者：
Barin, F;Jourdain, G;Lallemant, M
通讯作者：
Lallemant, M
影响因子：
4.2
作者：
Bodkin, C;Klopper, H;Langley, G
通讯作者：
Langley, G