Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer.
Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer.
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DOI：
10.1186/s13036-017-0074-3
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发表时间：
2017
影响因子：
5.6
通讯作者：
Hošek J
中科院分区：
文献类型：
--
作者：
Gregor A;Filová E;Novák M;Kronek J;Chlup H;Buzgo M;Blahnová V;Lukášová V;Bartoš M;Nečas A;Hošek J
组织工程的主要目的是再生或替换因疾病、损伤或先天性异常而受损的组织或器官。目前，组织工程学主要是通过人工支架来修复受损的组织和器官。这些用于适当细胞的附着和随后的生长。在细胞生长过程中，支架逐渐发生生物降解，最终产品是具有所需形状和特性的新组织。近年来，研究工作的重点是通过生物制造技术开发支架，以实现这些结构的快速，精确和廉价的自动制造。最有前途的技术似乎是快速成型，由于其高水平的精度和控制。然而，这种技术在容易用于支架制造之前仍然要解决各种问题。在这篇文章中，我们使用市售的设备和材料测试了临床适用支架的打印。本文介绍的研究一般集中在骨组织置换领域的“支架”上。商业上可获得的3D打印机和聚乳酸用于创建最初设计的并且可能适合骨组织工程的支架结构。我们测试了具有不同几何结构的支架的打印。基于骨肉瘤细胞增殖实验和设计的支架样品的机械测试，将说明可能没有必要将用于骨组织置换的支架的推荐孔隙率保持在约90%，并且还将阐明为什么这一事实消除了机械性能问题。此外，证明单个孔的尺寸可以是0.2-0.35 mm之间的推荐范围的尺寸的两倍，而不影响细胞增殖。基于熔融沉积成型的快速成型技术用于制造设计的支架结构。所有的实验都是为了展示如何可能解决目前研究工作场所在支架生物制造领域报告的某些限制和问题。这些结果将为进一步的研究提供新的有价值的知识。
The primary objective of Tissue engineering is a regeneration or replacement of tissues or organs damaged by disease, injury, or congenital anomalies. At present, Tissue engineering repairs damaged tissues and organs with artificial supporting structures called scaffolds. These are used for attachment and subsequent growth of appropriate cells. During the cell growth gradual biodegradation of the scaffold occurs and the final product is a new tissue with the desired shape and properties. In recent years, research workplaces are focused on developing scaffold by bio-fabrication techniques to achieve fast, precise and cheap automatic manufacturing of these structures. Most promising techniques seem to be Rapid prototyping due to its high level of precision and controlling. However, this technique is still to solve various issues before it is easily used for scaffold fabrication. In this article we tested printing of clinically applicable scaffolds with use of commercially available devices and materials. Research presented in this article is in general focused on “scaffolding” on a field of bone tissue replacement. Commercially available 3D printer and Polylactic acid were used to create originally designed and possibly suitable scaffold structures for bone tissue engineering. We tested printing of scaffolds with different geometrical structures. Based on the osteosarcoma cells proliferation experiment and mechanical testing of designed scaffold samples, it will be stated that it is likely not necessary to keep the recommended porosity of the scaffold for bone tissue replacement at about 90%, and it will also be clarified why this fact eliminates mechanical properties issue. Moreover, it is demonstrated that the size of an individual pore could be double the size of the recommended range between 0.2–0.35 mm without affecting the cell proliferation. Rapid prototyping technique based on Fused deposition modelling was used for the fabrication of designed scaffold structures. All the experiments were performed in order to show how to possibly solve certain limitations and issues that are currently reported by research workplaces on the field of scaffold bio-fabrication. These results should provide new valuable knowledge for further research.
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影响因子：
6.7
作者：
Heo DN;Castro NJ;Lee SJ;Noh H;Zhu W;Zhang LG
通讯作者：
Zhang LG
DOI：
10.1097/01.alc.0000118315.58404.c1
发表时间：
2004-03-01
影响因子：
3.2
作者：
Gong, ZD;Wezeman, FH
通讯作者：
Wezeman, FH
影响因子：
2.4
作者：
GIBSON, LJ
通讯作者：
GIBSON, LJ
DOI：
10.1016/j.jmbbm.2015.07.021
发表时间：
2015-11-01
影响因子：
3.9
作者：
Baldino, L.;Naddeo, F.;Reverchon, E.
通讯作者：
Reverchon, E.
影响因子：
3
作者：
Choee, JH;Lee, SJ;Khang, G
通讯作者：
Khang, G