Taccaoside induces apoptosis in hepatocellular carcinoma cell lines.
Taccaoside induces apoptosis in hepatocellular carcinoma cell lines.
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Taccaoside 诱导肝细胞癌细胞系凋亡
DOI：
10.1177/0300060516672368
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Liang G
中科院分区：
文献类型：
--
作者：
Sun Y;Qiu H;Ou M;Chen R;Liang G
塔考皂苷是一种类固醇皂苷，已被证明具有细胞毒性，但其细胞毒性机制尚不清楚。本研究观察了塔果皂苷对人肝癌细胞系SMMC-7721和Bel-7404的影响。用3-(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑溴化法测定其抗增殖作用。用Hoechst 33258染色观察细胞形态。流式细胞仪检测细胞周期和细胞凋亡率。半胱氨酸天冬氨酸氨基转移酶(Caspase)活性检测采用特异法，PARP、Bax、Bcl2表达分析采用Western blotting。Taccao side对肝癌细胞生长的抑制作用呈浓度和时间依赖关系。Taccao side使细胞周期停滞于G2/M期，并诱导caspase依赖的细胞凋亡。Western blotting显示，塔果皂苷可上调Bax的表达，下调Bcl2的表达。Taccao side治疗后观察到PARP的裂解。本研究表明，塔果皂苷可能通过诱导细胞凋亡来抑制肝癌细胞的增殖。
Taccaoside, a steroidal saponin, has been shown to be cytotoxic, although the mechanism of cytotoxicity remains unclear. This study examined the effect of taccaoside on the human hepatocellular carcinoma (HCC) cell lines SMMC-7721 and Bel-7404. The antiproliferative effect of taccaoside were measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Cells were stained with Hoechst 33258 to observe morphology. Cell cycle and apoptosis were analysed by flow cytometry. Caspase activation was detected using specific assays, and PARP, Bax and Bcl-2 expression were analysed using western blotting. Taccaoside showed antiproliferative effect on HCC cell lines growth in a concentration- and time-dependent manner. Taccaoside arrested cell cycle in the G2/M phase and induced caspase-dependent apoptosis. Western blotting indicated that taccaoside upregulated Bax expression and downregulated Bcl-2 expression. PARP cleavage was observed following taccaoside treatment. This study showed that taccaoside may inhibit HCC cell proliferation by inducing apoptosis.
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影响因子：
--
作者：
Brentnall M;Rodriguez-Menocal L;De Guevara RL;Cepero E;Boise LH
通讯作者：
Boise LH
DOI：
10.1007/bf00567287
发表时间：
1980-01-01
期刊：
Chemistry of Natural Compounds (English Translation of Khimiya Prirodnykh Soedinenii)
影响因子：
--
作者：
NGOK F K;KEL'GINBAEV A N;ABUBAKIROV N K
通讯作者：
ABUBAKIROV N K
影响因子：
2.8
作者：
Kontos, Christos K.;Christodoulou, Maria-Ioanna;Scorilas, Andreas
通讯作者：
Scorilas, Andreas
影响因子：
5.4
作者：
Long, Fang-Yi;Chen, Ya-Shu;Du, Jun-Rong
通讯作者：
Du, Jun-Rong
影响因子：
13.5
作者：
Altekruse, Sean F.;McGlynn, Katherine A.;Dickie, Lois A.;Kleiner, David E.
通讯作者：
Kleiner, David E.