Cell surface proteoglycan expression correlates with epithelial-mesenchymal interaction during tooth morphogenesis.
Cell surface proteoglycan expression correlates with epithelial-mesenchymal interaction during tooth morphogenesis.
复制标题
细胞表面蛋白多糖表达与牙齿形态发生过程中上皮-间质相互作用相关。
DOI：
10.1016/0012-1606(88)90401-0
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发表时间：
1988
影响因子：
2.7
通讯作者：
Merton Bernfield
中科院分区：
文献类型：
--
作者：
Irma Thesleff;Markku Jalkanen;Seppo Vainio;Merton Bernfield
牙齿的形态发生和牙细胞的分化是由上皮组织和间质组织之间的相互作用引导的。由于细胞外基质参与这些相互作用，因此位于细胞表面的基质受体的表达可能会在此发育过程中发生变化。我们检查了上皮细胞表面蛋白多糖抗原的分布，已知该抗原在牙齿形态发生过程中充当间质基质的受体。在胚胎口腔上皮细胞以及早期芽期的牙上皮细胞周围观察到强烈的染色。随着发育，釉质器官中的表达大大减少。这些细胞分化成成釉细胞与表达丧失相关，而保留在中间层和星状网中的上皮细胞则重新获得强烈的染色。 PG抗原在松散的神经嵴衍生的颌间充质中表达较弱，但当发生广泛的形态发生运动时，它在致密的牙乳头间充质中变得强烈反应。随着发育，PG抗原从晚期牙乳头间充质中消失，但持续存在于牙囊间充质中，从而形成牙周组织。成牙本质细胞不表达PG抗原。因此，PG抗原的表达在牙齿发育的上皮-间质相互作用过程中发生变化，并在终末细胞分化过程中丢失。该表达遵循形态发生而不是组织学边界。牙齿发育过程中上皮和间质组织表达的获得和丧失表明，这种蛋白多糖在指导形态发生的上皮间质相互作用中具有特定功能。
Tooth morphogenesis and differentiation of the dental cells are guided by interactions between epithelial and mesenchymal tissues. Because the extracellular matrix is involved in these interactions, the expression of matrix receptors located at the cell surface may change during this developmental sequence. We have examined the distribution of an epithelial cell surface proteoglycan antigen, known to behave as a receptor for interstitial matrix, during tooth morphogenesis. Intense staining was seen around the cells of the embryonic oral epithelium as well as the dental epithelium at the early bud stage. With development, expression was greatly reduced in the enamel organ. Differentiation of these cells into ameloblasts was associated with the loss of expression, while the epithelial cells remaining in the stratum intermedium and stellate reticulum regained intense staining. The PG antigen was weakly expressed in the loose neural crest-derived jaw mesenchyme but it became strongly reactive in the condensed dental papilla mesenchyme when extensive morphogenetic movements took place. With development, the PG antigen disappeared from the advanced dental papilla mesenchyme but persisted in the dental sac mesenchyme, which gives rise to periodontal tissues. The PG antigen was not expressed by odontoblasts. Hence, the expression of the PG antigen changes during the epithelial-mesenchymal interactions of tooth development and is lost during terminal cell differentiation. The expression follows morphogenetic rather than histologic boundaries. The acquisition and loss of expression in epithelial and mesenchymal tissues during tooth development suggest that this proteoglycan has specific functions in the epithelialmesenchymal interactions that guide morphogenesis.
来自小鼠乳腺上皮细胞的硫酸乙酰肝素蛋白多糖。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rapraeger,AC;Bernfield,M
通讯作者：
Bernfield,M
影响因子：
3.2
作者：
HAYASHI, K;HAYASHI, M;BERNFIELD, M
通讯作者：
BERNFIELD, M
来自小鼠乳腺上皮细胞的细胞表面蛋白聚糖含有硫酸软骨素和硫酸乙酰肝素糖胺聚糖。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rapraeger,A;Jalkanen,M;Endo,E;Koda,J;Bernfield,M
通讯作者：
Bernfield,M