Role of nanoparticle surface functionality in the disruption of model cell membranes.
Role of nanoparticle surface functionality in the disruption of model cell membranes.
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DOI：
10.1021/la302654s
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发表时间：
2012-11-27
期刊：
影响因子：
--
通讯作者：
Posner JD
中科院分区：
文献类型：
--
作者：
Moghadam BY;Hou WC;Corredor C;Westerhoff P;Posner JD
脂质双层是细胞生命中常见的生物膜，构成细胞与其环境之间的连续屏障。了解工程纳米材料（ENM）与脂质双层的相互作用是预测后续生物效应的重要一步。在这项研究中，我们评估了不同的表面功能和浓度的10 nm直径的金（Au）和二氧化钛（TiO 2）ENMs上的带负电荷的脂质双层囊泡（脂质体）使用染料泄漏试验的破坏的影响。我们的研究结果表明，具有正和负表面电荷的Au ENM诱导泄漏，在几个小时后达到稳定状态。具有相同的表面功能和不同的核心组成的带正电荷的颗粒显示出类似的泄漏效应，并导致比带负电荷的颗粒更快和更大的泄漏，这表明表面功能，而不是颗粒核心组成，是决定ENM和脂质双层之间相互作用的关键因素。结果表明，颗粒永久吸附到双层，并且仅需要一个带正电荷的颗粒来破坏脂质体并触发其全部内容物的泄漏，而蜂毒素分子是研究最广泛的膜溶解肽，其需要数百个分子来产生泄漏。
Lipid bilayers are biomembranes common to cellular life and constitute a continuous barrier between cells and their environment. Understanding the interaction of engineered nanomaterials (ENMs) with lipid bilayers is an important step toward predicting subsequent biological effects. In this study, we assess the effect of varying the surface functionality and concentration of 10 nm-diameter gold (Au) and titanium dioxide (TiO2) ENMs on the disruption of negatively charged lipid bilayer vesicles (liposomes) using a dye leakage assay. Our findings show that Au ENMs having both positive and negative surface charge induce leakage that reaches a steady state after several hours. Positively charged particles with identical surface functionality and different core composition show similar leakage effects and result in faster and greater leakage than negatively charged particles, which suggests that surface functionality, not particle core composition, is a critical factor in determining the interaction between ENMs and lipid bilayers. The results suggest that particles permanently adsorb to bilayers and that only one positively charged particle is required to disrupt a liposome and trigger leakage of its entire contents in contrast to mellitin molecules, the most widely studied membrane lytic peptide, which requires hundred of molecules to generate leakage.
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