Effect of Temperature on Topological States of Circular DNA
Effect of Temperature on Topological States of Circular DNA
复制标题
温度对环状 DNA 拓扑状态的影响
DOI：
10.1088/0253-6102/68/1/131
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发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
陈虎
中科院分区：
文献类型：
--
作者：
范洋涛;李秀燕;刘艳辉;陈虎
环状双链DNA的不同拓扑状态可以通过它们的连接数来定义。通过连接酶将线性DNA环化成圆形，可以得到链数的平衡分布。基于DNA弯曲刚度和扭转刚度对温度的强烈依赖性的实验结果，弯曲刚度的减小值可以在温度区间（5 ~ 53）°C内近似表示，扭转刚度的温度依赖关系可以用nm来拟合，环状DNA的连接数分布的温度依赖关系可以用蒙特卡罗模拟来预测。连接数分布随温度的变化与以往的实验结果一致。与弯曲刚度的温度依赖性相比，扭转刚度的温度依赖性会引起连接数分布的显著波动，并主要导致环状DNA连接数分布的方差变化。方程中扭曲数和扭曲数的方差取决于环状DNA的长度。当环状DNA长度小于230 nm时，扭转数的方差占主导地位，而当环状DNA长度大于370 nm时，扭转数的方差占主导地位。
The different topological states of circular double-stranded DNA can be defined by their linking number. The equilibrium distribution of linking number can be obtained by circularizing a linear DNA into a circle by ligase. Based on the recent experimental results that the DNA bending rigidity and twist rigidity strongly depend on temperature, the reduced bending rigidity can be approximated by over the temperature interval (5 ~ 53) °C, and the temperature dependence of twist rigidity can be fitted by nm. The temperature dependence of the linking number distribution of circular DNAs can be predicted by using Monte Carlo simulation. The variance of linking number distribution on temperature is in accordance with the previous experimental results. Compared with the temperature dependence of bending rigidity, the temperature dependence of twist rigidity causes a noticeable fluctuation in linking number distribution and mainly contribute towards the variance change of linking number distribution of circular DNA. The variance of the writhe number and twist number in the equation depends on the length of circular DNA. When the length of circular DNA is less than 230 nm, the variance of twist number is dominant over the variance of writhe number , whereas for the condition that the length of the circular DNA is larger than 370 nm.
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质粒DNA的拓扑行为。
影响因子：
3.7
作者：
Higgins NP;Vologodskii AV
通讯作者：
Vologodskii AV
DOI：
10.1146/annurev.bb.23.060194.003141
发表时间：
1994
期刊：
Annual review of biophysics and biomolecular structure
影响因子：
--
作者：
A. Vologodskii;N. Cozzarelli
通讯作者：
A. Vologodskii;N. Cozzarelli
DOI：
10.1073/pnas.68.4.815
发表时间：
1971-01-01
影响因子：
11.1
作者：
FULLER, FB
通讯作者：
FULLER, FB
影响因子：
5.5
作者：
Moroz, JD;Nelson, P
通讯作者：
Nelson, P
影响因子：
3.4
作者：
Christopher A. Sucato;D. Rangel;Dan Aspleaf;B. Fujimoto;J. Michael Schurr
通讯作者：
Christopher A. Sucato;D. Rangel;Dan Aspleaf;B. Fujimoto;J. Michael Schurr