Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels.

Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels.
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鉴定从聚丙烯酰胺凝胶转移至硝化纤维的组织特异性核抗原。

DOI:
10.1126/science.7003713
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发表时间:
1981
期刊:
Science (New York, N.Y.)
影响因子:
--
通讯作者:
Hnilica,LS
Hnilica,LS
中科院分区:
--
文献类型:
--
作者:
Glass2nd,WF;Briggs,RC;Hnilica,LS

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非组蛋白抗原经十二烷基硫酸钠电泳法分离后,转移到硝酸纤维素膜上,进行免疫化学鉴定。用抗血清去组化Novikoff肝癌染色质,与正常大鼠肝脏相比,发现了许多Novikoff肝癌染色质特异的蛋白质抗原。
Nonhistone protein antigens resolved by electrophoresis in sodium dodecyl sulfate were identified immunochemically after being transferred to nitrocellulose. Use of antiserum to dehistonized chromatin from Novikoff hepatoma revealed numerous protein antigens specific to the chromatin of Novikoff hepatoma in comparison to that of normal rat liver.
DOI: 10.1126/science.154.3757.1662
发表时间: 1966-01-01
期刊: SCIENCE
影响因子: 56.9
作者:
BLOBEL, G;POTTER, VR
通讯作者: POTTER, VR
DOI: --
发表时间: 1971
期刊: Biochimica et Biophysica Acta
影响因子: --
作者:
Thomas C. Spelsberg;Lubomir S. Hnilica;A. Ansevin
通讯作者: A. Ansevin