Replication of respiratory syncytial virus is inhibited by the host defense molecule viperin.
Replication of respiratory syncytial virus is inhibited by the host defense molecule viperin.
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DOI：
10.1159/000342473
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发表时间：
2013
影响因子：
5.3
通讯作者：
Bakaletz LO
中科院分区：
文献类型：
--
作者：
McGillivary G;Jordan ZB;Peeples ME;Bakaletz LO
呼吸道合胞病毒(RSV)是引起中耳炎、毛细支气管炎和肺炎的重要病原体。由于上呼吸道感染是这些疾病发展的先决条件，了解RSV的发病机制和在这个利基环境中诱导的宿主反应可能有助于开发针对这种病毒的新治疗策略。我们使用微阵列方法显示，在RSV攻击后一周，编码抗病毒蛋白Viperin的基因在栗鼠鼻咽中的表达显著上调。体外过表达人毒蛇蛋白可降低RSV感染HeLa或A549细胞的能力。此外，用编码蛇毒的重组腺相关病毒载体转导龙猫呼吸道，可降低鼻咽灌洗液中RSV的滴度。总而言之，这些数据表明，毒蛇在抵抗RSV的先天免疫防御中发挥着重要作用。
Respiratory syncytial virus (RSV) is an important viral pathogen of otitis media, bronchiolitis, and pneumonia. As infection of the upper airway is a precondition for development of these diseases, understanding RSV pathogenesis and the host response induced by RSV in this niche may enable development of novel therapeutic strategies against this virus. We have used a microarray approach and showed that expression of the gene that encodes the antiviral protein Viperin was significantly upregulated in the chinchilla nasopharynx up to a week after RSV challenge. Overexpression of human Viperin in vitro diminished the ability of RSV to infect HeLa or A549 cells. Furthermore, transduction of the chinchilla airway with a recombinant adeno-associated virus vector that encodes viperin resulted in reduced titers of RSV in nasopharyngeal lavage fluid. Collectively, these data indicated that Viperin plays a significant role in the innate immune defense against RSV.
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影响因子：
3.7
作者：
McGillivary G;Bakaletz LO
通讯作者：
Bakaletz LO
影响因子：
5.4
作者：
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通讯作者：
Durbin, JE
影响因子：
5.4
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影响因子：
5.4
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Brasier, Allan R.
影响因子：
5.4
作者：
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通讯作者：
Peeples, Mark E.