Adiponectin-induced secretion of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) is impaired in monocytes from patients with type I diabetes.
Adiponectin-induced secretion of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) is impaired in monocytes from patients with type I diabetes.
复制标题
脂联素诱导的白介素-6（IL-6），单核细胞趋化蛋白-1（MCP-1，CCL2）和白介素8（IL-8，CXCL8）的分泌受到I型糖尿病患者的单核细胞的损害。
DOI：
10.1186/1475-2840-5-17
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发表时间：
2006-08-30
影响因子：
9.3
通讯作者：
Buechler, Christa
中科院分区：
文献类型：
--
作者：
Abke, Sabine;Neumeier, Markus;Weigert, Johanna;Wehrwein, Gabriele;Eggenhofer, Elke;Schaeffler, Andreas;Maier, Kevin;Aslanidis, Charalampos;Schoelmerich, Juergen;Buechler, Christa
心血管疾病（CVD）患者全身脂联素水平降低，低脂联素水平可能参与动脉粥样硬化的发病机制。然而，循环脂联素在1型糖尿病（T1 D）患者中升高，这些患者也具有更高的CVD发病率。由于单核细胞在动脉粥样硬化中起重要作用，我们分析了脂联素对T1 D患者和对照组单核细胞中细胞因子和趋化因子释放的影响。测定血浆中的全身性脂联素，并通过免疫印迹法分析脂联素的高分子量（HMW）形式。从T1 D患者和对照中分离单核细胞，并分析脂联素刺激的白细胞介素-6（IL-6）、单核细胞趋化蛋白-1（MCP-1，CCL 2）和白细胞介素-8（IL-8，CXCL 8）的释放。T1 D患者的全身脂联素水平较高。血浆的免疫印迹分析表明T1 D患者和对照组中高分子量脂联素的丰度。IL-6，CCL 2和CXCL 8分泌在脂联素诱导单核细胞从控制，而只有IL-6上调T1 D细胞。NFκB通路抑制剂可阻断脂联素对IL-6的诱导作用。这些数据表明，脂联素介导的IL-6、CCL 2和CXCL 8的诱导在T1 D患者的单核细胞中受到干扰，因此与对照相比，T1 D患者中升高的全身脂联素可能保护性较低。
Systemic adiponectin is reduced in patients with cardiovascular disease (CVD) and low adiponectin may contribute to the pathogenesis of atherosclerosis. However, circulating adiponectin is elevated in type 1 diabetes (T1D) patients, who have also a higher incidence to develop CVD. Because monocytes play an important role in atherosclerosis, we analysed the influence of adiponectin on cytokine and chemokine release in monocytes from T1D patients and controls. Systemic adiponectin was determined in the plasma and the high-molecular weight (HMW) form of adiponectin was analysed by immunoblot. Monocytes were isolated from T1D patients and controls and the adiponectin-stimulated release of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) was analysed. Systemic adiponectin was higher in T1D patients. Immunoblot analysis of the plasma indicate abundance of HMW adiponectin in T1D patients and controls. IL-6, CCL2 and CXCL8 secretion in response to adiponectin were found induced in monocytes from controls whereas only IL-6 was upregulated in T1D cells. The induction of IL-6 by adiponectin was abrogated by an inhibitor of the NFκB pathway. These data indicate that adiponectin-mediated induction of IL-6, CCL2 and CXCL8 is disturbed in monocytes from T1D patients and therefore elevated systemic adiponectin in T1D patients may be less protective when compared to controls.
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影响因子：
4.6
作者：
Bouma, G;Coppens, JMC;Versnel, MA
通讯作者：
Versnel, MA
影响因子：
--
作者：
Geerlings, SE;Hoepelman, AIM
通讯作者：
Hoepelman, AIM
影响因子：
8.2
作者：
Frystyk, J;Tarnow, L;Flyvbjerg, A
通讯作者：
Flyvbjerg, A
影响因子：
4.4
作者：
Koukkunen, H;Penttilä, K;Pyörälä, K
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Pyörälä, K
影响因子：
4.4
作者：
Patterson, JE;Andriole, VT
通讯作者：
Andriole, VT