Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility.
Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility.
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DOI：
10.1111/nph.12408
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Vinatzer BA
中科院分区：
文献类型：
--
作者：
Clarke CR;Chinchilla D;Hind SR;Taguchi F;Miki R;Ichinose Y;Martin GB;Leman S;Felix G;Vinatzer BA
细菌鞭毛蛋白（FliC）表位flg 22和flgII-28是微生物相关分子模式（MAMP）。虽然flg 22通过模式识别受体FLS 2被许多植物物种识别，但flgII-28受体和flgII-28被不同植物科识别的程度都是未知的。在这里，我们测试了flgII-28作为MAMP的意义以及flg 22和flgII-28中等位基因多样性在植物-病原体相互作用中的重要性，使用纯化的肽和补充有不同fliC等位基因的假单胞菌ΔfliC突变体。植物基因型和等位基因的多样性flg 22和flgII-28被发现显着影响植物的免疫反应，但没有细菌的运动。flgII-28的识别仅限于一些茄科物种。虽然flgII-28肽在拟南芥中不引发任何免疫应答，但flg 22和flgII-28中的突变对毒力具有FLS 2依赖性影响。然而，FLS 2的番茄等位基因的表达并不赋予本氏烟草检测flgII-28的能力，并且FLS 2沉默的番茄植物在flgII-28识别方面没有改变。因此，MAMP多样化是一种有效的病原体毒力策略，并且flgII-28似乎被茄科中尚未鉴定的受体感知，尽管它在拟南芥中具有FLS 2依赖的毒力效应。
The bacterial flagellin (FliC) epitopes flg22 and flgII-28 are microbe-associated molecular patterns (MAMPs). While flg22 is recognized by many plant species via the pattern recognition receptor FLS2, neither the flgII-28 receptor nor the extent of flgII-28 recognition by different plant families is known. Here we tested the significance of flgII-28 as a MAMP and the importance of allelic diversity in flg22 and flgII-28 in plant–pathogen interactions using purified peptides and a Pseudomonas syringae ΔfliC mutant complemented with different fliC alleles. Plant genotype and allelic diversity in flg22 and flgII-28 were found to significantly affect the plant immune response but not bacterial motility. Recognition of flgII-28 is restricted to a number of Solanaceous species. While the flgII-28 peptide does not trigger any immune response in Arabidopsis, mutations in both flg22 and flgII-28 have FLS2-dependent effects on virulence. However, expression of a tomato allele of FLS2 does not confer to Nicotiana benthamiana the ability to detect flgII-28 and tomato plants silenced for FLS2 are not altered in flgII-28 recognition. Therefore, MAMP diversification is an effective pathogen virulence strategy and flgII-28 appears to be perceived by a yet unidentified receptor in the Solanaceae although it has an FLS2-dependent virulence effect in Arabidopsis.
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影响因子：
6.7
作者：
Baltrus DA;Nishimura MT;Romanchuk A;Chang JH;Mukhtar MS;Cherkis K;Roach J;Grant SR;Jones CD;Dangl JL
通讯作者：
Dangl JL
影响因子：
30.3
作者：
Cheng W;Munkvold KR;Gao H;Mathieu J;Schwizer S;Wang S;Yan YB;Wang J;Martin GB;Chai J
通讯作者：
Chai J
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
DOI：
10.1073/pnas.0502040102
发表时间：
2005-06-28
影响因子：
11.1
作者：
Andersen-Nissen, E;Smith, KD;Aderem, A
通讯作者：
Aderem, A
影响因子：
30.5
作者：
Ausubel, FM
通讯作者：
Ausubel, FM