Systematic exploration of Escherichia coli phage-host interactions with the BASEL phage collection.
Systematic exploration of Escherichia coli phage-host interactions with the BASEL phage collection.
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DOI：
10.1371/journal.pbio.3001424
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发表时间：
2021-11
期刊：
影响因子：
9.8
通讯作者：
Harms A
中科院分区：
文献类型：
--
作者：
Maffei E;Shaidullina A;Burkolter M;Heyer Y;Estermann F;Druelle V;Sauer P;Willi L;Michaelis S;Hilbi H;Thaler DS;Harms A
噬菌体是感染细菌的病毒，由于其无与伦比的多样性和基因工程的最新突破，在治疗多重耐药细菌感染和其他应用方面具有巨大的潜力。然而，噬菌体-宿主相互作用的分子机制的基础知识主要局限于一些传统的模型系统，并没有跟上最近的大规模扩张的领域。因此，由这些病毒编码的分子生物学的真正潜力在很大程度上仍未开发，并且用于治疗或其他应用的药物通常仍然是凭经验选择的。因此，我们试图通过组成一个由68个新分离的感染模式生物大肠杆菌的噬菌体组成的分类良好的文库来促进对噬菌体-宿主相互作用的系统探索，我们与社区共享该文库作为巴塞尔（为您的实验室选择噬菌体）集合。该系列在很大程度上代表了天然E。大肠杆菌噬菌体多样性，并集中表征表型和基因组一起10个研究良好的传统模式ESTA。我们通过实验确定了所有大肠杆菌的基本宿主受体，量化了它们对不同细菌免疫层的11种防御系统的敏感性，并将这些结果与一组致病性肠杆菌菌株的大肠杆菌宿主范围相匹配。噬菌体表型和基因组特征分布的清晰模式突出了不同免疫系统效力的系统差异，并表明了几个噬菌体组中受体特异性的分子基础。我们的研究结果还表明，健身性状之间的权衡，如广泛的主机识别和细菌免疫的阻力，可能会推动不同的噬菌体群的不同适应特定的生态位。我们设想，巴塞尔系列将激发未来的工作，探索噬菌体及其宿主的生物学，促进发现潜在的分子机制，作为有效翻译成生物技术或治疗应用的基础。本研究介绍了感染模式细菌大肠杆菌的细菌的巴塞尔收集;该社区的资源是天然大肠杆菌的代表。大肠杆菌噬菌体多样性，并已广泛表征的表型和基因组。
Bacteriophages, the viruses infecting bacteria, hold great potential for the treatment of multidrug-resistant bacterial infections and other applications due to their unparalleled diversity and recent breakthroughs in their genetic engineering. However, fundamental knowledge of the molecular mechanisms underlying phage–host interactions is mostly confined to a few traditional model systems and did not keep pace with the recent massive expansion of the field. The true potential of molecular biology encoded by these viruses has therefore remained largely untapped, and phages for therapy or other applications are often still selected empirically. We therefore sought to promote a systematic exploration of phage–host interactions by composing a well-assorted library of 68 newly isolated phages infecting the model organism Escherichia coli that we share with the community as the BASEL (BActeriophage SElection for your Laboratory) collection. This collection is largely representative of natural E. coli phage diversity and was intensively characterized phenotypically and genomically alongside 10 well-studied traditional model phages. We experimentally determined essential host receptors of all phages, quantified their sensitivity to 11 defense systems across different layers of bacterial immunity, and matched these results to the phages’ host range across a panel of pathogenic enterobacterial strains. Clear patterns in the distribution of phage phenotypes and genomic features highlighted systematic differences in the potency of different immunity systems and suggested the molecular basis of receptor specificity in several phage groups. Our results also indicate strong trade-offs between fitness traits like broad host recognition and resistance to bacterial immunity that might drive the divergent adaptation of different phage groups to specific ecological niches. We envision that the BASEL collection will inspire future work exploring the biology of bacteriophages and their hosts by facilitating the discovery of underlying molecular mechanisms as the basis for an effective translation into biotechnology or therapeutic applications. This study presents the BASEL collection of phages that infect the model bacterium Escherichia coli; this resource for the community is representative of natural E. coli phage diversity and has been extensively characterized phenotypically and genomically.
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DOI：
10.3390/v9040070
发表时间：
2017-04-03
期刊：
Viruses
影响因子：
--
作者：
Adriaenssens E;Brister JR
通讯作者：
Brister JR
DOI：
10.1007/978-1-4939-7343-9_15
发表时间：
2018-01-01
期刊：
BACTERIOPHAGES, VOL. 3
影响因子：
--
作者：
Aziz, Ramy Karam;Ackermann, Hans-Wolfgang;Kropinski, Andrew M.
通讯作者：
Kropinski, Andrew M.
影响因子：
3.1
作者：
Amor, K;Heinrichs, DE;Whitfield, C
通讯作者：
Whitfield, C
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--
影响因子：
9.3
作者：
Ando, Hiroki;Lemire, Sebastien;Lu, Timothy K.
通讯作者：
Lu, Timothy K.