Increased Lipids in Chlamydomonas reinhardtii by Multiple Regulations of DOF, LACS2, and CIS1.
Increased Lipids in Chlamydomonas reinhardtii by Multiple Regulations of DOF, LACS2, and CIS1.
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DOF、LACS2 和 CIS1 的多重调控增加了莱茵衣藻中的脂质
DOI：
10.3390/ijms231710176
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发表时间：
2022-09-05
影响因子：
5.6
通讯作者：
Huang, Ying
中科院分区：
文献类型：
--
作者：
Jia, Bin;Yin, Jianbo;Li, Xiaolian;Li, Yingling;Yang, Xingcai;Lan, Chengxiang;Huang, Ying
关键词：
微藻脂质对于生物燃料和膳食补充剂的生产至关重要。用于提高产量的脂质工程已经研究多年。然而，由于脂质代谢的复杂性，单基因工程逐渐遇到瓶颈。多基因调控更有利于促进脂质积累，进一步阐明脂质生物合成在脂质、碳水化合物和蛋白质代谢稳态中的复杂调控机制。在这里，三个脂质相关基因 DOF、LACS2 和 CIS 在莱茵衣藻中通过两个循环的转化共同调控，以过表达 DOF 并同时敲低 LACS2 和 CIS。在这些基因的多重调控下，与CC849相比，细胞内脂质和FA含量分别增加了142%和52%，而淀粉和蛋白质含量则减少了45%和24%。转录组分析显示，TAG和FA生物合成基因上调，淀粉和蛋白质代谢基因下调。这表明更多来自淀粉和蛋白质代谢的碳前体通量被重定向到脂质合成途径。这些结果表明，调节各种代谢中的基因有助于碳通量重定向并显着改善细胞内脂质，证明了多种基因调节策略的潜力，并为微藻中脂质过量生产提供了可能的候选者。
Microalgal lipids are essential for biofuel and dietary supplement production. Lipid engineering for higher production has been studied for years. However, due to the complexity of lipid metabolism, single-gene engineering gradually encounters bottlenecks. Multiple gene regulation is more beneficial to boosting lipid accumulation and further clarifying the complex regulatory mechanism of lipid biosynthesis in the homeostasis of lipids, carbohydrates, and protein metabolism. Here, three lipid-related genes, DOF, LACS2, and CIS, were co-regulated in Chlamydomonas reinhartii by two circles of transformation to overexpress DOF and knock down LACS2 and CIS simultaneously. With the multiple regulations of these genes, the intracellular lipids and FA content increased by 142% and 52%, respectively, compared with CC849, whereas the starch and protein contents decreased by 45% and 24%. Transcriptomic analysis showed that genes in TAG and FA biosynthesis were up-regulated, and genes in starch and protein metabolism were down-regulated. This revealed that more carbon precursor fluxes from starch and protein metabolism were redirected towards lipid synthesis pathways. These results showed that regulating genes in various metabolisms contributed to carbon flux redirection and significantly improved intracellular lipids, demonstrating the potential of multiple gene regulation strategies and providing possible candidates for lipid overproduction in microalgae.
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影响因子：
5.6
作者：
Munoz, Camilo F.;Weusthuis, Ruud A.;Wijffels, Rene H.
通讯作者：
Wijffels, Rene H.
影响因子：
7.2
作者：
Molnar, Attila;Bassett, Andrew;Baulcombe, David
通讯作者：
Baulcombe, David
影响因子：
6.9
作者：
Pick U;Avidan O
通讯作者：
Avidan O
影响因子：
3.7
作者：
Rasala BA;Lee PA;Shen Z;Briggs SP;Mendez M;Mayfield SP
通讯作者：
Mayfield SP
影响因子：
4.8
作者：
Boyle, Nanette R.;Page, Mark Dudley;Merchant, Sabeeha S.
通讯作者：
Merchant, Sabeeha S.