May viral load detected in saliva in the early stages of infection be a prognostic indicator in COVID-19 patients?
May viral load detected in saliva in the early stages of infection be a prognostic indicator in COVID-19 patients?
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DOI：
10.1016/j.jviromet.2021.114198
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发表时间：
2021-08
影响因子：
3.1
通讯作者：
Geckil AA
中科院分区：
文献类型：
--
作者：
Aydin S;Benk IG;Geckil AA
本研究旨在探讨新冠肺炎感染早期唾液病毒载量检测对预后判断的价值。所有患者均于新冠肺炎发病早期同时采集口腔鼻咽拭子和唾液标本。从经RT-qPCR检测SARS-CoV-2阳性的患者唾液中提取病毒RNA，检测病毒载量。记录并比较两组患者的人口学资料、合并症、周期阈值和一个月的临床病程。对患者的临床病程进行1个月的评估，其中轻度疾病占56%，中度疾病占26.4%，重度疾病占9.6%，危重/死亡疾病占8%。唾液和咽喉标本中SARS-CoV-2的平均周期阈值分别为22.28和24.19。唾液循环阈值对疾病严重程度的预测意义显著(Eta系数0.979)。在疾病的严重程度和ONP样本的Ct值的平均值之间发现有统计学意义的关系(p<；0.05)。性别、年龄、体重指数和合并症与疾病的严重程度进行了比较；没有发现统计学上的显著差异。在新冠肺炎感染的早期阶段从唾液中检测到的病毒载量在显示45岁以上患者的病程方面可能具有预后价值。唾液是新冠肺炎筛查的一种容易获得、可靠的材料。
This study aimed to investigate the prognostic value of viral load detected in the saliva of COVID-19 patients in the early stage of infection. Oro-nasopharyngeal swab and saliva samples were collected from all patients simultaneously in the early stage of COVID-19. Viral loads were determined by extracting viral RNAs from saliva samples of patients whose ONP swabs were positive for SARS-CoV-2 by RT-qPCR. The demographic information, comorbidities, cycle threshold values, and one-month clinical courses were recorded and compared. The patients' clinical course was evaluated for one month; 56 % of patients had mild disease, 26.4 % had moderate disease, 9.6 % had severe disease, and 8% had a critical/mortal disease. The average cycle threshold values of SARS-CoV-2 in saliva and ONP samples were measured as 22.28 and 24.19, respectively. Cycle threshold value of saliva was found to be significant in predicting disease severity (Eta coefficient 0.979). A statistically significant relationship was found between the disease's severity and the mean of ONP samples’ Ct-values (p < 0.05). Gender, age, body mass index, and co-morbidities were compared with the severity of the disease; no statistically significant difference was found. Viral load detected in saliva in the early period of COVID-19 infection may have a prognostic value in showing the disease's course in patients over 45-year-old. Saliva is an easily obtainable, reliable material for COVID-19 screening.
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