Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts.
Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts.
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DOI：
10.1038/s41467-020-19925-0
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发表时间：
2020-12-03
影响因子：
16.6
通讯作者：
Carvalho VM
中科院分区：
文献类型：
--
作者：
Cardozo KHM;Lebkuchen A;Okai GG;Schuch RA;Viana LG;Olive AN;Lazari CDS;Fraga AM;Granato CFH;Pintão MCT;Carvalho VM
严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）的爆发给世界各地的公共卫生系统带来了压力，大规模人群检测是控制这一大流行性疾病的关键步骤。在这里，我们开发了一种高通量的靶向蛋白质组学检测SARS-CoV-2核蛋白肽直接从鼻咽和口咽拭子。在机器人液体处理器上实施的改良的基于磁性颗粒的蛋白质组学方法能够在4小时内完全自动化制备96个样品。TFC-MS系统可在10分钟内对4个样品进行多重分析，每天可处理500多个样品。我们使用先前通过实时RT-PCR分析的985份标本定性（第3层）和定量（第1层）验证了该方法，并检测出高达84%的阳性病例，特异性高达97%。所提出的策略具有较高的样本稳定性，应被视为在大人群中进行SARS-CoV-2检测的一种选择。大规模人群检测是控制COVID-19疫情的关键一步。在这里，作者开发了一种基于肽的SARS-CoV-2检测的靶向质谱系统，允许每天分析500多个拭子样本，即使在室温下长时间储存样本后也能检测病毒。
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pressing public health systems around the world, and large population testing is a key step to control this pandemic disease. Here, we develop a high-throughput targeted proteomics assay to detect SARS-CoV-2 nucleoprotein peptides directly from nasopharyngeal and oropharyngeal swabs. A modified magnetic particle-based proteomics approach implemented on a robotic liquid handler enables fully automated preparation of 96 samples within 4 hours. A TFC-MS system allows multiplexed analysis of 4 samples within 10 min, enabling the processing of more than 500 samples per day. We validate this method qualitatively (Tier 3) and quantitatively (Tier 1) using 985 specimens previously analyzed by real-time RT-PCR, and detect up to 84% of the positive cases with up to 97% specificity. The presented strategy has high sample stability and should be considered as an option for SARS-CoV-2 testing in large populations. Large population testing is a key step to controlling the COVID-19 pandemic. Here, the authors develop a targeted mass spectrometry system for peptide-based SARS-CoV-2 detection, allowing analysis of over 500 swab samples per day and enabling virus detection even after prolonged sample storage at room temperature.
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