Recent Advances and Ongoing Challenges in the Diagnosis of Microbial Infections by MALDI-TOF Mass Spectrometry.
Recent Advances and Ongoing Challenges in the Diagnosis of Microbial Infections by MALDI-TOF Mass Spectrometry.
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DOI：
10.3389/fmicb.2018.01097
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发表时间：
2018
影响因子：
5.2
通讯作者：
Lupetti A
中科院分区：
文献类型：
--
作者：
Florio W;Tavanti A;Barnini S;Ghelardi E;Lupetti A
微生物感染诊断的及时性和准确性与降低死亡率和缩短住院时间有关，特别是对危及生命的严重感染。快速诊断还允许早期简化经验性抗微生物疗法，从而有助于限制抗微生物药物耐药性的出现和传播。基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法（MS）的引入对微生物病原体的常规鉴定产生了深远的影响，并正在逐步取代生物化学鉴定方法。与目前使用的鉴定方法相比，MALDI-TOF MS的优点是可以在几分钟内直接从培养板上培养的菌落中鉴定细菌和酵母，并进行初级分离，节省了大量的材料和劳动力。MALDI-TOF MS在临床相关细菌和酵母菌鉴定中的可靠性和准确性已被多项研究证明，MALDI-TOF MS的性能与目前临床微生物实验室使用的表型方法相当或优于，并且可以通过数据库更新和分析软件升级进一步提高。除了从分离菌落中鉴定微生物外，MALDI-TOF质谱还在探索新的前景，例如直接从阳性血培养中鉴定病原体、亚种分型和检测耐药决定因素。本文综述了MALDI-TOF质谱在微生物病原体常规鉴定中的研究进展，并重点介绍了该技术在菌株分型、药敏评估和毒力因子检测等特殊应用方面的最新进展。
Timeliness and accuracy in the diagnosis of microbial infections are associated with decreased mortality and reduced length of hospitalization, especially for severe, life-threatening infections. A rapid diagnosis also allows for early streamlining of empirical antimicrobial therapies, thus contributing to limit the emergence and spread of antimicrobial resistance. The introduction of matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) for routine identification of microbial pathogens has profoundly influenced microbiological diagnostics, and is progressively replacing biochemical identification methods. Compared to currently used identification methods, MALDI-TOF MS has the advantage of identifying bacteria and yeasts directly from colonies grown on culture plates for primary isolation in a few minutes and with considerable material and labor savings. The reliability and accuracy of MALDI-TOF MS in identification of clinically relevant bacteria and yeasts has been demonstrated by several studies showing that the performance of MALDI-TOF MS is comparable or superior to phenotypic methods currently in use in clinical microbiology laboratories, and can be further improved by database updates and analysis software upgrades. Besides microbial identification from isolated colonies, new perspectives are being explored for MALDI-TOF MS, such as identification of pathogens directly from positive blood cultures, sub-species typing, and detection of drug resistance determinants. In this review, we summarize the state of the art in routine identification of microbial pathogens by MALDI-TOF MS, and highlight recent advancements of this technology in special applications, such as strain typing, assessment of drug susceptibility, and detection of virulence factors.
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影响因子：
7.4
作者：
Du, ZM;Yang, RF;Wang, J
通讯作者：
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基质辅助激光解吸电离飞行时间质谱法鉴定分枝杆菌的准确性：系统评价和荟萃分析。
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作者：
Barnini S;Ghelardi E;Brucculeri V;Morici P;Lupetti A
通讯作者：
Lupetti A
影响因子：
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作者：
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通讯作者：
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