Effects and mechanisms of apolipoprotein A-V on the regulation of lipid accumulation in cardiomyocytes.
Effects and mechanisms of apolipoprotein A-V on the regulation of lipid accumulation in cardiomyocytes.
复制标题
载脂蛋白A-V对心肌细胞脂质积累调节的作用及机制
DOI：
10.1186/s12944-018-0692-x
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发表时间：
2018-03-12
影响因子：
4.5
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Luo J;Xu L;Li J;Zhao S
载脂蛋白（apo） a - v是甘油三酯（TG）代谢的关键调节器。本研究探讨apoA-V对心肌细胞脂质代谢的影响。方法首先研究apoA-V是否可以被心肌细胞摄取，以及低密度脂蛋白受体家族成员是否参与这一过程。接下来，检测不同浓度apoA-V在正常和肥胖动物的脂质堆积细胞中甘油三酯（TG）含量和脂滴的变化。最后，我们测试了通过[14C]-油酸进入心肌细胞的脂肪酸（FAs）水平和脂质分泌。结果心脏组织中存在apoA-V蛋白，apoA-V被心肌细胞吸收。当HL-1细胞转染低密度脂蛋白受体（LDLR）相关蛋白1(LRP1) siRNA时，apoA-V摄入量减少53% (P<0.05)，而HL-1细胞中37%的脂质积累保持不变。ApoA-V定位于细胞质，并与HL-1细胞的脂滴相关。1200和1800 ng/mL apoA-V干预使HL-1细胞中TG含量分别降低28%和45%，使小鼠心脏组织中TG含量降低39% （P<0.05）。然而，apoA-V对正常HL-1细胞和小鼠的TG含量均无影响。心肌细胞吸收FAs水平降低43% (P < 0.05)， TG和胆固醇酯分泌水平分别升高1.2倍和1.6倍（P < 0.05）。结论apoa - v是一种新的心肌细胞脂质代谢调节因子。
BackgroundApolipoprotein (apo) A-V is a key regulator of triglyceride (TG) metabolism. We investigated effects of apoA-V on lipid metabolism in cardiomyocytes in this study.MethodsWe first examined whether apoA-V can be taken up by cardiomyocytes and whether low density lipoprotein receptor family members participate in this process. Next, triglyceride (TG) content and lipid droplet changes were detected at different concentrations of apoA-V in normal and lipid-accumulation cells in normal and obese animals. Finally, we tested the levels of fatty acids (FAs) taken up into cardiomyocytes and lipid secretion through [14C]-oleic acid.ResultsOur results show that heart tissue has apoA-V protein, and apoA-V is taken up by cardiomyocytes. When HL-1 cells were transfected with low density lipoprotein receptor (LDLR)-related protein 1(LRP1) siRNA, apoA-V intake decreased by 53% (P<0.05), while a 37% lipid accumulation in HL-1 cells remain unchanged. ApoA-V localized to the cytoplasm and was associated with lipid droplets in HL-1 cells. A 1200 and 1800 ng/mL apoA-V intervention decreased TG content by 28% and 45% in HL-1 cells, respectively and decreased TG content by 39% in mouse heart tissue (P<0.05). However, apoA-V had no effects on TG content in either normal HL-1 cells or mice. The levels of FAs taken up into cardiomyocytes decreased by 43% (P < 0.05), and the levels of TG and cholesterol ester secretion increased by 1.2-fold and 1.6-fold, respectively (P < 0.05).ConclusionApoA-V is a novel regulator of lipid metabolism in cardiomyocytes.
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影响因子：
4.8
作者：
Christoffersen, C;Bollano, E;Nielsen, LB
通讯作者：
Nielsen, LB
DOI：
10.1152/ajpheart.00113.2002
发表时间：
2002-10-01
影响因子：
4.8
作者：
Lamberts, RR;Van Rijen, MHP;Westerhof, N
通讯作者：
Westerhof, N
雌激素通过调节肝脏 APOA5 表达降低甘油三酯
影响因子：
4.5
作者：
Luo F;Guo Y;Ruan GY;Peng R;Li XP
通讯作者：
Li XP
影响因子：
10.8
作者：
Cal, Roi;Castellano, Jose;Llorente-Cortes, Vicenta
通讯作者：
Llorente-Cortes, Vicenta
影响因子：
4.8
作者：
Carley, Andrew N.;Kleinfeld, Alan M.
通讯作者：
Kleinfeld, Alan M.