HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium.
HERG potassium channels are more frequently expressed in human endometrial cancer as compared to non-cancerous endometrium.
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DOI：
10.1054/bjoc.2000.1497
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发表时间：
2000-12
影响因子：
8.8
通讯作者：
Arcangeli, A
中科院分区：
文献类型：
--
作者：
Cherubini, A;Taddei, GL;Crociani, O;Paglierani, M;Buccoliero, AM;Fontana, L;Noci, I;Borri, P;Borrani, E;Giachi, M;Becchetti, A;Rosati, B;Wanke, E;Olivotto, M;Arcangeli, A
HERG K+通道，除了有助于调节心脏和神经元的兴奋性，优先在不同组织发生的肿瘤细胞系中表达，其在肿瘤表型的发展和维持中的作用正在研究中。我们在这里表明，HERG基因和HERG蛋白表达的高频率在原发性人类子宫内膜癌，相比正常和增生的子宫内膜。RT-PCR和免疫组化，使用我们实验室开发的特异性抗HERG抗体，应用于从18个子宫内膜癌和11个非癌子宫内膜组织中获得的组织标本。HERG RNA和HERG蛋白分别在67%和82%癌组织中表达，而仅在18%的非癌组织中表达。特别是在子宫内膜增生中未发现表达。此外，电生理学实验证实了肿瘤细胞质膜上存在功能性HERG通道。总的来说，这些数据是HERG通道在原发性人类肿瘤中存在的第一个证明，并且可以候选HERG作为能够标记癌性与增生性子宫内膜生长的潜在工具。© 2000年癌症研究运动http://www.bjcancer.com
HERG K+channels, besides contributing to regulate cardiac and neuronal excitability, are preferentially expressed in tumour cell lines of different histogenesis, where their role in the development and maintenance of the neoplastic phenotype is under study. We show here that both herg gene and HERG protein are expressed with high frequency in primary human endometrial cancers, as compared to normal and hyperplastic endometrium. RT-PCR and immunohistochemistry, using specific anti-HERG antibodies developed in our laboratory, were applied to tissue specimens obtained from 18 endometrial cancers and 11 non-cancerous endometrial tissues. herg RNA and HERG protein are expressed in 67% and 82%, respectively, of cancerous, while in only 18% of non-cancerous tissues. In particular, no expression was found in endometrial hyperplasia. Moreover, electrophysiological experiments confirmed the presence of functioning HERG channels on the plasma membrane of tumour cells. On the whole, these data are the first demonstration of the presence of HERG channels in primary human neoplasias, and could candidate HERG as a potential tool capable of marking cancerous versus hyperplastic endometrial growth. © 2000 Cancer Research Campaign http://www.bjcancer.com
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影响因子：
4.8
作者：
Pond, AL;Scheve, BK;Nerbonne, JM
通讯作者：
Nerbonne, JM
影响因子：
5.5
作者：
Chiesa, N;Rosati, B;Wanke, E
通讯作者：
Wanke, E
影响因子：
11.4
作者：
Pardo, LA;del Camino, D;Stühmer, W
通讯作者：
Stühmer, W
影响因子：
5.5
作者：
Faravelli, L;Arcangeli, A;Wanke, E
通讯作者：
Wanke, E
DOI：
10.1006/bbrc.1998.8319
发表时间：
1998-03-27
影响因子：
3.1
作者：
Arcangeli, A;Rosati, B;Olivotto, M
通讯作者：
Olivotto, M