Hepatic Vein Blood Increases Lung Microvascular Angiogenesis and Endothelial Cell Survival-Toward an Understanding of Univentricular Circulation.
Hepatic Vein Blood Increases Lung Microvascular Angiogenesis and Endothelial Cell Survival-Toward an Understanding of Univentricular Circulation.
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DOI：
10.1053/j.semtcvs.2020.03.004
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发表时间：
2020
影响因子：
2.5
通讯作者：
Ramchandran R
中科院分区：
文献类型：
--
作者：
Spearman AD;Gupta A;Pan AY;Gronseth EI;Thirugnanam K;Gudausky TM;Foerster SR;Ramchandran R
为了提高我们对单心室先天性心脏病肺动静脉畸形的认识，我们的目标是确定肝静脉和上腔静脉成分对肺微血管内皮细胞的影响，与血流无关。从接受心导管插入术的 0-10 岁儿童的肝静脉和上腔静脉采集配对血样。随后将分离的血清用于体外内皮细胞测定。使用管形成和划痕迁移来评估血管生成活性。使用增殖（BrdU 掺入、细胞周期分析）和凋亡（Caspase 3/7 活性、Annexin-V 标记）评估内皮细胞存活率。使用 Wilcoxon 符号秩检验和重复测量分析来分析数据。将肺微血管内皮细胞与 10% 患者血清一起孵育后，肝静脉血清会增加血管生成活性（管形成，p=0.04，n=24；迁移，p<0.001，n=18），增加增殖（BrdU，p<0.001，n=32；S 期，p=0.04，n=13），并减少细胞凋亡（半胱天冬酶 3/7，与上腔静脉血清相比，p<0.001，n=32；膜联蛋白-V，p=0.04，n=12。肝静脉血清通过增加体外血管生成和存活来调节肺微血管内皮细胞。肺微血管系统中肝静脉血清信号传导的丧失可能会促进适应不良的肺微血管重塑和肺动静脉畸形。
To improve our understanding of pulmonary arteriovenous malformations in univentricular congenital heart disease, our objective was to identify the effects of hepatic vein and superior vena cava constituents on lung microvascular endothelial cells independent of blood flow. Paired blood samples were collected from the hepatic vein and superior vena cava in children 0-10 years-old undergoing cardiac catheterization. Isolated serum was subsequently used for in vitro endothelial cell assays. Angiogenic activity was assessed using tube formation and scratch migration. Endothelial cell survival was assessed using proliferation (BrdU incorporation, cell cycle analysis) and apoptosis (caspase 3/7 activity, Annexin-V labeling). Data were analyzed using Wilcoxon signed-rank test and repeated measures analysis. Upon incubating lung microvascular endothelial cells with 10% patient serum, hepatic vein serum increases angiogenic activity (tube formation, p=0.04, n=24; migration, p<0.001, n=18), increases proliferation (BrdU, p<0.001, n=32; S-phase, p=0.04, n=13), and decreases apoptosis (caspase 3/7, p<0.001, n=32; Annexin-V, p=0.04, n=12) compared to superior vena cava serum. Hepatic vein serum regulates lung microvascular endothelial cells by increasing angiogenesis and survival in vitro. Loss of hepatic vein serum signaling in the lung microvasculature may promote maladaptive lung microvascular remodeling and pulmonary arteriovenous malformations.
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DOI：
10.1016/j.jtcvs.2010.08.088
发表时间：
2011-06-01
影响因子：
6
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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DOI：
10.1016/j.jtcvs.2008.02.011
发表时间：
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影响因子：
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通讯作者：
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