Consensus guidelines for the use and interpretation of angiogenesis assays.

Consensus guidelines for the use and interpretation of angiogenesis assays.
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DOI:
10.1007/s10456-018-9613-x
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发表时间:
2018-08
期刊:
影响因子:
9.8
通讯作者:
Griffioen AW
Griffioen AW
中科院分区:
医学1区
文献类型:
--
作者:
Nowak-Sliwinska P;Alitalo K;Allen E;Anisimov A;Aplin AC;Auerbach R;Augustin HG;Bates DO;van Beijnum JR;Bender RHF;Bergers G;Bikfalvi A;Bischoff J;Böck BC;Brooks PC;Bussolino F;Cakir B;Carmeliet P;Castranova D;Cimpean AM;Cleaver O;Coukos G;Davis GE;De Palma M;Dimberg A;Dings RPM;Djonov V;Dudley AC;Dufton NP;Fendt SM;Ferrara N;Fruttiger M;Fukumura D;Ghesquière B;Gong Y;Griffin RJ;Harris AL;Hughes CCW;Hultgren NW;Iruela-Arispe ML;Irving M;Jain RK;Kalluri R;Kalucka J;Kerbel RS;Kitajewski J;Klaassen I;Kleinmann HK;Koolwijk P;Kuczynski E;Kwak BR;Marien K;Melero-Martin JM;Munn LL;Nicosia RF;Noel A;Nurro J;Olsson AK;Petrova TV;Pietras K;Pili R;Pollard JW;Post MJ;Quax PHA;Rabinovich GA;Raica M;Randi AM;Ribatti D;Ruegg C;Schlingemann RO;Schulte-Merker S;Smith LEH;Song JW;Stacker SA;Stalin J;Stratman AN;Van de Velde M;van Hinsbergh VWM;Vermeulen PB;Waltenberger J;Weinstein BM;Xin H;Yetkin-Arik B;Yla-Herttuala S;Yoder MC;Griffioen AW

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新血管的形成或血管生成是一个复杂的过程,在生长发育、组织和器官再生以及许多病理条件中起着重要作用。血管生成经历了多个离散的步骤,这些步骤可以通过大量的生物测定来单独评估和量化。这些独立评估有优点,但也有局限性。本文描述了可用于评估血管生成的体内、离体和体外生物测定,并强调了与其执行和正确解释相关的关键方面。因此,这项合作工作是血管生成生物测定共识指南的第一版,为当前和未来提供参考。
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
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