Association between Food Intake, Clinical and Metabolic Markers and DNA Damage in Older Subjects.
Association between Food Intake, Clinical and Metabolic Markers and DNA Damage in Older Subjects.
复制标题
食物摄入，临床和代谢标志物与老年受试者的DNA损伤之间的关联。
DOI：
10.3390/antiox10050730
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发表时间：
2021-05-06
期刊：
影响因子：
--
通讯作者：
Riso P
中科院分区：
文献类型：
--
作者：
Del Bo' C;Martini D;Bernardi S;Gigliotti L;Marino M;Gargari G;Meroño T;Hidalgo-Liberona N;Andres-Lacueva C;Kroon PA;Cherubini A;Guglielmetti S;Porrini M;Riso P
使用 DNA 损伤作为氧化应激、代谢功能障碍和年龄相关疾病的标志存在争议。本研究旨在评估参加 MaPLE（肠道和血液微生物组学，用于研究富含多酚的饮食模式对老年人肠道通透性的影响）干预试验的一组具有肠道通透性的老年受试者的 DNA 损伤水平（评估为 DNA 链断裂、内源性和氧化诱导的 DNA 损伤），以评估其与临床、代谢和饮食标志物的关联。通过彗星试验对参与该研究的 49 名老年受试者进行了外周血单核细胞 DNA 损伤的评估。测定血清中的临床和代谢标志物、炎症标志物、血管功能和肠道通透性。食物摄入量通过加权食物日记来估计。总体而言，与女性相比，男性 DNA 损伤水平呈更高水平的趋势 (p = 0.071)。 DNA 损伤与临床/代谢标志物（例如尿酸、血脂）之间存在正相关关系，而与饮食标志物（例如维生素 C、E、B6、叶酸）之间存在负相关关系，并且这种关联因性别而异。通过考虑衰老过程中 DNA 稳定性的重要性，关于性别差异以及饮食和代谢因素对 DNA 损伤的潜在作用所获得的结果强调需要在更大的老年人群体中进行进一步研究，以确认所发现的关联并促进预防策略。
The use of DNA damage as marker of oxidative stress, metabolic dysfunction and age-related diseases is debated. The present study aimed at assessing the level of DNA damage (evaluated as DNA strand-breaks, endogenous and oxidatively-induced DNA damage) in a group of older subjects with intestinal permeability enrolled within the MaPLE (Gut and Blood Microbiomics for Studying the Effect of a Polyphenol-Rich Dietary Pattern on Intestinal Permeability in the Elderly) intervention trial, to evaluate its association with clinical, metabolic and dietary markers. DNA damage in peripheral blood mononuclear cells was assessed by the comet assay in 49 older subjects participating in the study. Clinical and metabolic markers, markers of inflammation, vascular function and intestinal permeability were determined in serum. Food intake was estimated by weighted food diaries. On the whole, a trend towards higher levels of DNA damage was observed in men compared to women (p = 0.071). A positive association between DNA damage and clinical/metabolic markers (e.g., uric acid, lipid profile) and an inverse association with dietary markers (e.g., vitamin C, E, B6, folates) were found and differed based on sex. By considering the importance of DNA stability during aging, the results obtained on sex differences and the potential role of dietary and metabolic factors on DNA damage underline the need for further investigations in a larger group of older adults to confirm the associations found and to promote preventive strategies.
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