Postnatal reorganization of actin filaments and differentiation of intercellular boundaries in the rat aortic endothelial cells
Postnatal reorganization of actin filaments and differentiation of intercellular boundaries in the rat aortic endothelial cells
复制标题
大鼠主动脉内皮细胞肌动蛋白丝的出生后重组和细胞间边界的分化
DOI：
10.1007/bf00331365
复制
发表时间：
1994
影响因子：
3.6
通讯作者：
T. Sakai
中科院分区：
文献类型：
--
作者：
N. Kobayashi;T. Sakai
用罗丹明-鬼笔环肽染色和激光共聚焦显微镜观察了大鼠主动脉内皮细胞肌动蛋白丝分布的生后变化。大鼠主动脉内皮细胞具有两个群体的肌动蛋白丝束，即在细胞边缘的周边带和应力纤维在细胞质中纵向运行。新生大鼠的主动脉内皮细胞仅含有应力纤维，而10天龄大鼠的主动脉内皮细胞既有外周带又有应力纤维。20日龄后，主动脉内皮细胞主要具有外周带，偶尔在分支孔周围有应力纤维。在出生后的发展过程中，主动脉内皮细胞的应力纤维的长度密度下降，而个别应力纤维在内皮细胞缩短。电子显微镜观察显示，出生时的主动脉内皮细胞的高细胞间边界的高度下降，并在20天后的年龄发展的细胞质相互交织。在细胞边界处的外周带的出现被认为与细胞质交错的形成密切相关，其加强了内皮细胞之间的机械连接以对抗增加的跨壁压。新生大鼠主动脉内皮细胞应力纤维的表达可能受到发育中主动脉纵向伸长的影响，而生后其表达的减少则与主动脉内皮细胞所承受的流体切应力的变化有关。
Postnatal change in the distribution of actin filaments in endothelial cells was studied in the rat aorta by use of rhodamine-phalloidin staining and confocal laser scanning microscopy. Endothelial cells of the rat aorta possessed two populations of actin filament bundles, namely, peripheral bands at the cell border and stress fibers running longitudinally in the cytoplasm. Aortic endothelial cells of the neonatal rat contained only stress fibers, whereas those of the 10-day-old rat developed both peripheral bands and stress fibers. After 20 days of age, aortic endothelial cells had predominantly peripheral bands with occasional stress fibers around the branch orifices. During postnatal development the length density of stress fibers in aortic endothelial cells decreased, whereas individual stress fibers in endothelial cells were shortened. Electron-microscopic observation revealed that the high intercellular boundaries of aortic endothelial cells at birth decreased in height and developed cytoplasmic interdigitations after 20 days of age. The occurrence of peripheral bands at the cell border is thought to be closely related to formation of cytoplasmic interdigitation which strengthens the mechanical connection between endothelial cells against increasing transmural pressure. Expression of stress fibers in aortic endothelial cells of the neonatal rat is supposed to be affected by longitudinal elongation of the developing aorta, whereas their postnatal decrease is though to be correlated with the change of fluid shear stress loaded in the aortic endothelium.
循环拉伸对不同血管床内皮细胞的影响。
DOI：
--
发表时间：
1991
期刊：
Circulatory shock
影响因子：
--
作者：
Iba,T;Maitz,S;Furbert,T;Rosales,O;Widmann,MD;Spillane,B;Shin,T;Sonoda,T;Sumpio,BE
通讯作者：
Sumpio,BE
DOI：
10.1115/1.2891226
发表时间：
1991-05-01
影响因子：
1.7
作者：
HELMLINGER, G;GEIGER, RV;NEREM, RM
通讯作者：
NEREM, RM