Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension.
Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension.
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DOI：
10.1038/s41598-017-08259-5
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发表时间：
2017-08-10
影响因子：
4.6
通讯作者：
Suetsugu S
中科院分区：
文献类型：
--
作者：
Tachikawa M;Morone N;Senju Y;Sugiura T;Hanawa-Suetsugu K;Mochizuki A;Suetsugu S
小窝是丰富的瓶状内陷的质膜，缓冲膜张力通过其变形。很少有定量研究的变形窖的报道。每个小窝含有大约150个小窝蛋白-1。在这项研究中，我们通过测量投射到二维（2D）平面上的小窝蛋白-1的密度来估计小窝变形的程度。假设扁平小窝中的小窝蛋白-1具有烧瓶状小窝中的小窝蛋白-1的密度的大约四分之一。通过低渗处理增加质膜张力后，1/4密度小窝蛋白-1的比例增加。四分之一密度的小窝蛋白-1可溶于洗涤剂，并在等渗培养下与细胞小窝中的小窝蛋白-1形成连续的群体。不同的，分散的低密度小窝蛋白-1可溶于洗涤剂和增加后的张力的应用程序，这表明低渗透压张力诱导小窝蛋白-1的分散从小窝，可能通过扁平的小窝中间体。
Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.
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影响因子：
48
作者：
Dempsey, Graham T.;Vaughan, Joshua C.;Chen, Kok Hao;Bates, Mark;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
DOI：
10.1080/19490992.2015.1128604
发表时间：
2015
期刊：
Bioarchitecture
影响因子：
--
作者：
Senju Y;Suetsugu S
通讯作者：
Suetsugu S
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
56.9
作者：
Bates, Mark;Huang, Bo;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
4.8
作者：
Moltchanov, D.
通讯作者：
Moltchanov, D.