Human adipose stem cells maintain proliferative, synthetic and multipotential properties when suspension cultured as self-assembling spheroids.
Human adipose stem cells maintain proliferative, synthetic and multipotential properties when suspension cultured as self-assembling spheroids.
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当悬浮培养为自组装球体时，人类脂肪干细胞保持增殖、合成和多向特性
DOI：
10.1088/1758-5082/4/2/025004
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发表时间：
2012-06
期刊：
影响因子：
9
通讯作者：
Katz AJ
中科院分区：
文献类型：
--
作者：
Kapur SK;Wang X;Shang H;Yun S;Li X;Feng G;Khurgel M;Katz AJ
脂肪来源的基质/干细胞（ASCs）作为组织工程中极为通用的细胞来源已经得到认可。当ASCs作为三维细胞聚集体或球体培养时，我们展示了这些细胞的独特特性，进一步增强了ASCs在生物制造中的实用性。如本文所述，三维配方或自组装ASC球体发展出自己的细胞外基质，用于增加细胞对机械应力的稳健性。细胞外基质的组成可以根据球体的外部环境而改变，这些结构可以以可重复的方式生长并保持一致的大小。球体配方有助于保持ASCs的生存能力和发育可塑性，即使在确定的无血清培养基条件下。我们首次表明，由这些球体产生的多代贴壁ASCs保留了向多种细胞/组织类型分化的能力。这些已证明的特性支持了培养扩增的ASCs是“器官打印”的优秀候选细胞材料的观点——从标准化的细胞“墨水”或细胞配方中开发复杂组织结构的方法。
Adipose-derived stromal/stem cells (ASCs) have been gaining recognition as an extremely versatile cell source for tissue engineering. The usefulness of ASCs in biofabrication is further enhanced by our demonstration of the unique properties of these cells when they are cultured as three-dimensional cellular aggregates or spheroids. As described herein, three-dimensional formulations, or self-assembling ASC spheroids develop their own extracellular matrix that serves to increase the robustness of the cells to mechanical stresses. The composition of the extracellular matrix can be altered based on the external environment of the spheroids and these constructs can be grown in a reproducible manner and to a consistent size. The spheroid formulation helps preserve the viability and developmental plasticity of ASCs even under defined, serum-free media conditions. For the first time, we show that multiple generations of adherent ASCs produced from these spheroids retain their ability to differentiate into multiple cell/tissue types. These demonstrated properties support the idea that culture-expanded ASCs are an excellent candidate cellular material for ‘organ printing’—the approach of developing complex tissue structures from a standardized cell ‘ink’ or cell formulation.
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影响因子：
3.8
作者：
Kelm, JM;Timmins, NE;Nielsen, LK
通讯作者：
Nielsen, LK
DOI：
10.1073/pnas.1735463100
发表时间：
2003-10-28
影响因子：
11.1
作者：
Levenberg, S;Huang, NF;Langer, R
通讯作者：
Langer, R
影响因子：
15.9
作者：
Layer, PG;Robitzki, A;Willbold, E
通讯作者：
Willbold, E
影响因子：
--
作者：
Fukuda, J;Nakazawa, K
通讯作者：
Nakazawa, K
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP