Regional astrogliosis in the mouse hypothalamus in response to obesity.
Regional astrogliosis in the mouse hypothalamus in response to obesity.
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DOI：
10.1002/cne.23233
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发表时间：
2013-04-15
影响因子：
2.5
通讯作者：
Ellacott, Kate L. J.
中科院分区：
文献类型：
--
作者：
Buckman, Laura B.;Thompson, Misty M.;Moreno, Heidi N.;Ellacott, Kate L. J.
肥胖与外周组织中的慢性低度炎症相关，这有助于胰岛素抵抗和心血管疾病等共病的发展。虽然不太广泛的特点，肥胖症也促进中枢神经系统（CNS）的炎症和CNS功能的这种炎症的后果才开始被检查。在对CNS损伤如炎症的反应中，星形胶质细胞经历称为反应性星形胶质细胞增生的肥大和增生过程。我们使用免疫组织化学方法检测星形胶质细胞标记物胶质细胞酸性蛋白（GFAP）在饮食诱导或遗传性肥胖小鼠脑中的差异分布，并与其各自的瘦对照组进行比较，以确定下丘脑不同核团是否显示出对肥胖反应的相似星形胶质细胞增生。瘦和肥胖动物之间显示最高差异GFAP免疫反应性的区域包括内侧视前核、室旁核和背内侧核。相比之下，在腹内侧核、下丘脑外侧区或下丘脑前部区很少见到星形胶质细胞增生。在肥胖动物中，高水平的GFAP免疫反应性通常与微血管有关。在饮食诱导的肥胖模型与遗传模型相比，肥胖动物与其瘦对照之间GFAP染色的差异分布没有差异。肥胖症中星形胶质细胞增生的确切原因尚不清楚。肥胖导致与微血管相关的GFAP免疫反应性升高的独特模式的发现表明星形胶质细胞增生可能是对血脑屏障和/或外周循环变化的反应。
Obesity is associated with chronic low-grade inflammation in peripheral tissues, which contributes to the development of comorbidities such as insulin resistance and cardiovascular disease. While less extensively characterized, obesity also promotes inflammation in the central nervous system (CNS) and the consequences of this inflammation for CNS function are only beginning to be examined. In response to CNS insults such as inflammation, astrocytes undergo a process of hypertrophy and hyperplasia known as reactive astrogliosis. We used immunohistochemistry to examine the differential distribution of the astrocyte marker glial-fibrillary acidic protein (GFAP) in the brains of diet-induced or genetically obese mice compared with their respective lean controls to determine whether different nuclei of the hypothalamus showed comparable astrogliosis in response to obesity. The areas that showed the highest differential GFAP immunoreactivity between lean and obese animals include the medial preoptic, paraventricular, and dorsomedial nuclei. Comparatively, little astrogliosis was seen in the ventromedial nucleus, lateral hypothalamus, or anterior hypothalamic area. In obese animals high levels of GFAP immunoreactivity were often associated with the microvasculature. There were no differences in the differential distribution of GFAP staining between obese animals and their lean controls in the diet-induced compared with the genetic model of obesity. The exact cause(s) of the astrogliosis in obesity is not known. The finding that obesity causes a distinct pattern of elevated GFAP immunoreactivity associated with microvessels suggests that the astrogliosis may be occurring as a response to changes at the blood–brain barrier and/or in the peripheral circulation.
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影响因子：
3.3
作者：
Pistell PJ;Morrison CD;Gupta S;Knight AG;Keller JN;Ingram DK;Bruce-Keller AJ
通讯作者：
Bruce-Keller AJ
DOI：
10.1016/j.bbadis.2008.10.004
发表时间：
2009-05
影响因子：
6.2
作者：
Bruce-Keller, Annadora J.;Keller, Jeffrey N.;Morrison, Christopher D.
通讯作者：
Morrison, Christopher D.
影响因子：
105.7
作者：
Whitmer, RA;Gunderson, EP;Yaffe, K
通讯作者：
Yaffe, K
下丘脑Ikkbeta/nf-kappab以及ER应力的育儿与能量不平衡和肥胖。
影响因子：
64.5
作者：
Zhang, Xiaoqing;Zhang, Guo;Zhang, Hai;Karin, Michael;Bai, Hua;Cai, Dongsheng
通讯作者：
Cai, Dongsheng
DOI：
10.1097/01.ta.0000200842.19740.38
发表时间：
2006-09-01
影响因子：
--
作者：
Brown, Carlos V. R.;Rhee, Peter;Demetriades, Demetrios
通讯作者：
Demetriades, Demetrios