Strigolactone regulates shoot development through a core signalling pathway.
Strigolactone regulates shoot development through a core signalling pathway.
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DOI：
10.1242/bio.021402
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发表时间：
2016-12-15
期刊：
影响因子：
2.4
通讯作者：
Leyser O
中科院分区：
文献类型：
--
作者：
Bennett T;Liang Y;Seale M;Ward S;Müller D;Leyser O
Strigolactone是最近发现的一类激素，调节植物发育的多个方面。DARF14(D14)α/β折叠蛋白已被鉴定为一种Strigolacone受体，可通过SCFMAX2泛素连接酶发挥作用，但这一机制的普遍性尚不清楚。已有多种蛋白质被认为是Strigolacone信号传递的靶点，包括SCFMAX2的直接蛋白水解靶和下游靶标。然而，在许多情况下，这些蛋白质与斯特里内酯信号的相关性和重要性还没有完全确定。在这里，我们评估了这些靶标在成体枝条发育反应中对Strigolacone信号的贡献。我们发现所有被研究的斯特里内酯反应都受到SCFMAX2和D14的调节，而不是受其他D14样蛋白的调节。我们进一步表明，所有被检查的斯特里内酯反应可能依赖于SMXL6分支中SMXL蛋白的降解，而不是其他建议的蛋白降解靶标BES1或Dellas。综上所述，我们的结果表明，在成体枝条中，Strigolacone信号转导的主要方式是D14启动的、Max2介导的SMXL6相关蛋白的降解。我们证实了BRANCHED1转录因子和PIN-FORMED1生长素外流载体是该途径调控枝条分枝的可能下游靶标，并表明BRC1可能与PIN1平行作用。摘要：Strigolactones通过D14发出信号，通过靶向SMXL6分支蛋白，而不是BES1或Della蛋白进行降解，从而调节新梢的发育。BRc1和Pin1似乎可以向下游作用来调节分支。
Strigolactones are a recently identified class of hormone that regulate multiple aspects of plant development. The DWARF14 (D14) α/β fold protein has been identified as a strigolactone receptor, which can act through the SCFMAX2 ubiquitin ligase, but the universality of this mechanism is not clear. Multiple proteins have been suggested as targets for strigolactone signalling, including both direct proteolytic targets of SCFMAX2, and downstream targets. However, the relevance and importance of these proteins to strigolactone signalling in many cases has not been fully established. Here we assess the contribution of these targets to strigolactone signalling in adult shoot developmental responses. We find that all examined strigolactone responses are regulated by SCFMAX2 and D14, and not by other D14-like proteins. We further show that all examined strigolactone responses likely depend on degradation of SMXL proteins in the SMXL6 clade, and not on the other proposed proteolytic targets BES1 or DELLAs. Taken together, our results suggest that in the adult shoot, the dominant mode of strigolactone signalling is D14-initiated, MAX2-mediated degradation of SMXL6-related proteins. We confirm that the BRANCHED1 transcription factor and the PIN-FORMED1 auxin efflux carrier are plausible downstream targets of this pathway in the regulation of shoot branching, and show that BRC1 likely acts in parallel to PIN1. Summary: Strigolactones signal through D14 to regulate shoot development by targeting SMXL6-clade proteins, but not BES1 or DELLA proteins, for degradation. BRC1 and PIN1 plausibly act downstream to regulate branching.
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