Neurotrophin-3 is involved in the formation of apical dendritic bundles in cortical layer 2 of the rat.
Neurotrophin-3 is involved in the formation of apical dendritic bundles in cortical layer 2 of the rat.
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DOI：
10.1093/cercor/bhp093
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发表时间：
2010-01
期刊：
影响因子：
--
通讯作者：
Rockland KS
中科院分区：
文献类型：
--
作者：
Miyashita T;Wintzer M;Kurotani T;Konishi T;Ichinohe N;Rockland KS
来自锥体神经元的顶端树突束是皮质神经元的显著特征，但具有显著的区域特化。在这里，我们调查的束形成的机制，重点放在层（L）2束在大鼠颗粒压后皮质（GRS），边缘区牵连的空间记忆。通过使用微阵列，我们首先搜索在出生后第3天（P）的GRS L2与P12的GRS L2（分别在束形成之前和之后）、与GRS L5（P3）以及与桶场皮质（BF）中的L2（P3）中高度特异性表达的基因。几个基因，包括神经营养因子-3（NT-3），被确定为短暂和特异性表达在GRS L2。其中三个被克隆并通过原位杂交证实。为了测试NT-3介导的事件是因果关系参与束的形成，我们使用在子宫内电穿孔过表达NT-3在其他皮质区。这产生了源于BF中L2神经元的突出树突束，其中L2束通常不存在。细胞内生物胞素填充，在体外生理记录后，显示BF的L1树突状分支增加。树突状束的控制异位诱导确定了NT-3的新作用，并为研究树突状束及其形成提供了一种新的体内模型。
Apical dendritic bundles from pyramidal neurons are a prominent feature of cortical neuropil but with significant area specializations. Here, we investigate mechanisms of bundle formation, focusing on layer (L) 2 bundles in rat granular retrosplenial cortex (GRS), a limbic area implicated in spatial memory. By using microarrays, we first searched for genes highly and specifically expressed in GRS L2 at postnatal day (P) 3 versus GRS L2 at P12 (respectively, before and after bundle formation), versus GRS L5 (at P3), and versus L2 in barrel field cortex (BF) (at P3). Several genes, including neurotrophin-3 (NT-3), were identified as transiently and specifically expressed in GRS L2. Three of these were cloned and confirmed by in situ hybridization. To test that NT-3–mediated events are causally involved in bundle formation, we used in utero electroporation to overexpress NT-3 in other cortical areas. This produced prominent bundles of dendrites originating from L2 neurons in BF, where L2 bundles are normally absent. Intracellular biocytin fills, after physiological recording in vitro, revealed increased dendritic branching in L1 of BF. The controlled ectopic induction of dendritic bundles identifies a new role for NT-3 and a new in vivo model for investigating dendritic bundles and their formation.
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影响因子：
2.9
作者：
FELDMAN, ML;PETERS, A
通讯作者：
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影响因子：
3.4
作者：
Hanamura, K;Harada, A;Yamamoto, N
通讯作者：
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3.4
作者：
Ichinohe, N;Yoshihara, Y;Rockland, KS
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影响因子：
15.9
作者：
Arikkath, Jyothi;Reichardt, Louis F.
通讯作者：
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影响因子：
5.3
作者：
Ichinohe, N;Fujiyama, F;Rockland, KS
通讯作者：
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