Mechanoregulation of intracellular Ca2+ in human autosomal recessive polycystic kidney disease cyst-lining renal epithelial cells.
Mechanoregulation of intracellular Ca2+ in human autosomal recessive polycystic kidney disease cyst-lining renal epithelial cells.
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人常染色体隐性多囊肾病囊肿衬里肾上皮细胞中细胞内 Ca2 的机械调节。
DOI：
10.1152/ajprenal.00341.2007
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Satlin,LisaM
中科院分区：
文献类型：
--
作者：
Rohatgi,Rajeev;Battini,Lorenzo;Kim,Paul;Israeli,Sharon;Wilson,PatriciaD;Gusella,GLuca;Satlin,LisaM
Mutations of cilia-expressed proteins are associated with an attenuated shear-induced increase in intracellular Ca2+concentration ([Ca2+]i) in renal epithelial cell lines derived from murine models of autosomal recessive polycystic kidney disease (ARPKD). We hypothesized that human ARPKD cyst-lining renal epithelial cells also exhibited dysregulated mechanosensation. To test this, conditionally immortalized cell lines derived from human fetal ARPKD cyst-lining (pool and clone 5E) cell lines with low levels of fibrocystin/polyductin expression and age-matched normal collecting tubule [human fetal collecting tubule (HFCT) pool and clone 2C] cell lines were grown in culture, loaded with a Ca2+indicator dye, and subjected to laminar shear. Clonal cell lines were derived from single cells present in pools of cells from cyst-lining and collecting tubules, microdissected from human kidney. Resting and peak [Ca2+]iwere similar between ARPKD 5E and pool, and HFCT 2C and pool; however, the flow-induced peak [Ca2+]iwas greater in ARPKD 5E (700 ± 87 nM,n= 21) than in HFCT 2C (315 ± 58 nM,n= 12;P< 0.01) cells. ARPKD 5E cells treated with Gd3+, an inhibitor of nonselective cation channels, inhibited but did not abolish the shear-induced [Ca2+]itransient. Cilia were ∼20% shorter in ARPKD than HFCT cells, but no difference in ciliary localization or total cellular expression of polycystin-2, a mechanosenory Gd3+-sensitive cation channel, was detected between ARPKD and HFCT cells. The intracellular Ca2+stores were similar between cells. In summary, human ARPKD cells exhibit an exaggerated Gd3+-sensitive mechano-induced Ca2+response compared with controls; whether this represents dysregulated polycystin-2 activity in ARPKD cells remains to be explored.
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影响因子：
56.9
作者：
Mochizuki, T;Wu, GQ;Somlo, S
通讯作者：
Somlo, S
影响因子：
13.6
作者：
Smith, Laurie A.;Bukanov, Nikolay O.;Ibraghimov-Beskrovnaya, Oxana
通讯作者：
Ibraghimov-Beskrovnaya, Oxana
影响因子：
3.3
作者：
Taulman, PD;Haycraft, CJ;Yoder, BK
通讯作者：
Yoder, BK
DOI：
10.1152/ajprenal.00273.2001
发表时间：
2002-03-01
影响因子：
4.2
作者：
Yoder, BK;Tousson, A;Balkovetz, DF
通讯作者：
Balkovetz, DF
影响因子：
13.6
作者：
Nauli, Surya M.;Rossetti, Sandro;Zhou, Jing
通讯作者：
Zhou, Jing