Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study
Fluorescence detection of deep intramucosal cancer excited by green light for photodynamic diagnosis using protoporphyrin IX induced by 5-aminolevulinic acid: an ex vivo study
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使用 5-氨基乙酰丙酸诱导的原卟啉 IX 进行绿光激发的深部粘膜内癌荧光检测以进行光动力诊断：一项离体研究
DOI：
10.1117/1.jbo.25.6.063809
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发表时间：
2020
影响因子：
3.5
通讯作者：
Awazu Kunio
中科院分区：
文献类型：
--
作者：
Ihara Daisuke;Hazama Hisanao;Nishimura Takahiro;Morita Yoshinori;Awazu Kunio
原卟啉IX（Protoporphyrin IX，PpIX）光动力学诊断（photodynamic diagnosis，PDD）对胃癌的诊断深度有限，导致在筛查和手术中漏诊粘膜内癌。我们研究了一个激发波长，可以扩展诊断深度的PpIX荧光在粘膜substrate.Approach：通过计算的深度依赖强度的激发光在猪胃粘膜的每个波长，波长之间的关系，荧光团的深度，和荧光强度进行了评估和荧光图像的PpIX小球位于不同的荧光团的深度进行了比较实验通过改变激发波长。在荧光观察中，在405 nm处，荧光团深度为0和1.0 mm处的荧光强度分别为5.4 × 103arb和1.0 × 103arb。在505nm处的平均值分别为5.3 × 101arb和1.9 × 102arb。结论：505 nm激发光可扩大PpIX PDD对粘膜内癌的诊断深度。
Significance:The diagnostic depth of photodynamic diagnosis (PDD) for gastric cancer with protoporphyrin IX (PpIX) is limited, which leads to missing intramucosal cancers in screening and surgery.Aim:The reason is that the excitation light, whose wavelength is determined by the highest absorption peak of PpIX (∼405 nm), is strongly attenuated by mucosal tissues. We investigated an excitation wavelength that can extend the diagnostic depth of PpIX fluorescence at the mucosal subsurface.Approach:By calculating the depth-dependent intensity of the excitation light in porcine gastric mucosa for each wavelength, relationships among the wavelength, fluorophore depth, and fluorescence intensity were assessed and fluorescence images of PpIX pellets located at different fluorophore depths were compared experimentally by changing the excitation wavelength.Results:The numerical calculation showed that a 505-nm excitation light provided the highest fluorescence intensities at a fluorophore depth deeper than 1.1 mm. In the fluorescence observation, the fluorescence intensities at fluorophore depths of 0 and 1.0 mm at 405 nm were 5.4 × 103and 1.0 × 103arb. units, whereas those at 505 nm were 5.3 × 101and 1.9 × 102arb. units, respectively.Conclusion:The experimental results suggest that the diagnosis depth of PDD with PpIX for intramucosal cancer can be extended by 505-nm excitation light.
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