CsSWEET2, a Hexose Transporter from Cucumber (Cucumis sativus L.), Affects Sugar Metabolism and Improves Cold Tolerance in Arabidopsis.
CsSWEET2, a Hexose Transporter from Cucumber (Cucumis sativus L.), Affects Sugar Metabolism and Improves Cold Tolerance in Arabidopsis.
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CSSWEET2是来自黄瓜（黄瓜Sativus L.）的己糖转运蛋白，会影响糖代谢并提高拟南芥的冷耐受性。
DOI：
10.3390/ijms23073886
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发表时间：
2022-03-31
影响因子：
5.6
通讯作者：
He H
中科院分区：
文献类型：
--
作者：
Hu L;Zhang F;Song S;Yu X;Ren Y;Zhao X;Liu H;Liu G;Wang Y;He H
糖是植物体内重要的渗透调节物质和信号分子，糖最终将被输出转运蛋白(SCETS)，它们构成了一个新的糖转运蛋白家族，在植物对多种非生物胁迫的反应中发挥着核心作用。本研究对黄瓜甜味基因家族成员CsSWEET2进行了鉴定和鉴定。β-葡萄糖醛酸苷酶在转基因拟南芥植株中的组织化学分析表明，CsSWEET2在叶片中高表达，亚细胞定位表明CsSWEET2蛋白定位于质膜和内质网。在酵母中的异源表达分析表明，CsSWEET2编码一个能量不依赖的己糖/H+单转运蛋白，可以补充葡萄糖和果糖运输的缺陷。与野生型拟南芥相比，高表达CsSWEET2的转基因拟南芥具有更低的相对电解质渗漏水平和更强的抗寒性。糖含量分析表明，转基因拟南芥植株的葡萄糖和果糖水平显著高于野生型植株。综上所述，我们的结果表明，CsSWEET2通过调节糖的代谢和区域化，在提高植物的耐冷性方面发挥了重要作用。
Sugars, which are critical osmotic compounds and signalling molecules in plants, and Sugars Will Eventually be Exported Transporters (SWEETs), which constitute a novel family of sugar transporters, play central roles in plant responses to multiple abiotic stresses. In the present study, a member of the SWEET gene family from cucumber (Cucumis sativus L.), CsSWEET2, was identified and characterized. Histochemical analysis of β-glucuronidase expression in transgenic Arabidopsis plants showed that CsSWEET2 is highly expressed in the leaves; subcellular localization indicated that CsSWEET2 proteins are localized in the plasma membrane and endoplasmic reticulum. Heterologous expression assays in yeast demonstrated that CsSWEET2 encodes an energy-independent hexose/H+ uniporter that can complement both glucose and fructose transport deficiencies. Compared with wild-type Arabidopsis plants, transgenic Arabidopsis plants overexpressing CsSWEET2 had much lower relative electrolyte leakage levels and were much more resistant to cold stress. Sugar content analysis showed that glucose and fructose levels in the transgenic Arabidopsis plants were significantly higher than those in the wild-type plants. Taken together, our results suggest that, by mediating sugar metabolism and compartmentation, CsSWEET2 plays a vital role in improving plant cold tolerance.
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影响因子：
14.8
作者：
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通讯作者：
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影响因子：
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