Ovarian Localization of Immunoglobulin G and Inhibin α-Subunit in Guinea Pigs after Passive Immunization against the Inhibin α-Subunit
Ovarian Localization of Immunoglobulin G and Inhibin α-Subunit in Guinea Pigs after Passive Immunization against the Inhibin α-Subunit
复制标题
豚鼠被动免疫抑制素 α 亚基后免疫球蛋白 G 和抑制素 α 亚基的卵巢定位
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Taya
中科院分区：
文献类型：
--
作者：
F. Shi;K. Mochida;O. Suzuki;J. Matsuda;A. Ogura;M. Ozawa;G. Watanabe;A. Suzuki;K. Taya
.本实验旨在研究免疫球蛋白G（IG G）和卵泡素α亚基被动免疫后豚鼠卵巢的定位及卵泡发育。通过植入孕激素管使实验室豚鼠同步发情。取出孕酮植入物后，给豚鼠静脉注射1.0 ml抗血清或正常山羊血清（NGS）。结果表明，IG G主要进入有腔卵泡的卵泡液，提示抗卵泡素抗体可能是影响豚鼠卵泡发育的局部调节因子。结果还表明，被动免疫可增加排卵前卵泡数和血浆雌二醇浓度，但不改变血浆促卵泡激素（FSH）浓度和用抗卵泡素抗血清染色的卵泡数。本研究首次报道了在卵泡内注射抗卵泡素抗血清后IG G的分布，证实了我们先前的假设，即只有优势卵泡才能被卵泡素α亚基染色。结果还提示，单次注射抗卵泡刺激素抗血清不能提高豚鼠排卵率。
. The experiment was conducted to elucidate ovarian localization of immunoglobulin G (Ig G) and inhibin α -subunit and follicular development in guinea pigs after passive immunization against the inhibin α -subunit. Estrus was synchronized in laboratory guinea pigs by implanting progesterone containing-tubes. After removal of the progesterone implants, the guinea pigs were injected i.v. with 1.0 ml inhibin antiserum or normal goat serum (NGS). The results showed that Ig G mainly entered the follicular fluid of antral follicles, indicating that possibly inhibin antibodies could be a local regulator affecting follicular development in guinea pigs. The results also showed that passive immunization against inhibin increased the number of follicles and plasma concentrations of estradiol before ovulation, but did not change the plasma concentrations of follicle-stimulating hormone (FSH) or the numbers of follicles stained with inhibin antiserum. The present study first reports that the distribution of Ig G after injections of inhibin antiserum and confirms our previous hypothesis that only dominant follicles could be stained for inhibin α -subunit. The results also imply that only one injection of inhibin antiserum could not increase the ovulation rate in guinea pigs.
影响因子：
4.8
作者：
RIVIER, C;VALE, W
通讯作者：
VALE, W