Bioinspired silicification of silica-binding peptide-silk protein chimeras: comparison of chemically and genetically produced proteins.
Bioinspired silicification of silica-binding peptide-silk protein chimeras: comparison of chemically and genetically produced proteins.
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DOI：
10.1021/bm201555c
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发表时间：
2012-03-12
影响因子：
6.2
通讯作者：
Perry, Carole C.
中科院分区：
文献类型：
--
作者：
Canabady-Rochelle, Laetitia L. S.;Belton, David J.;Deschaume, Olivier;Currie, Heather A.;Kaplan, David L.;Perry, Carole C.
通过遗传或化学方法合成了由“丝”和二氧化硅结合肽（KSLSRHDHIHHH）构成的新型蛋白质嵌合体，并评估了它们对二氧化硅-丝基嵌合体复合物形成的影响。从在N末端融合有一个二氧化硅结合肽的棒足络新妇蜘蛛丝（[SGRGLGGQG AGAAAAAGGA GQGGYGGLGSQG]n）的32个氨基酸共有序列的6个或15个重复构建遗传嵌合体。对于化学嵌合体，在通过重氮偶联和EDC/NHS活化所有酸基团修饰酪氨酸基团后，将25当量的二氧化硅结合肽化学偶联至天然桑蚕丝。在温和的生物材料相容性条件下形成二氧化硅后，探索了肽添加对丝和嵌合丝-二氧化硅复合材料的性质的影响。复合生物材料的性质可能与二氧化硅缩合的程度和与遗传衍生的变体相比，化学嵌合体中二氧化硅结合位点的数量更高有关。在所有情况下，溶液中蛋白质/嵌合体的结构决定了用二氧化硅沉积方法形成的复合结构的类型，其对丝基材料的二级结构组成几乎没有影响。类似于我们对含有R5肽（SSKKSGSYSGSKGSKRRIL）的基于遗传丝的嵌合体的研究，本研究中使用的嵌合体（遗传和化学）的作用更多地在于聚集和支架而不是催化缩合。肽身份、丝构建体（共有重复序列或丝源的数量）和合成方法（遗传或化学）的变量可用于“调节”所形成的复合材料的性质，并且是可用于制备用于生物医学和基于传感器的应用的一系列材料的通用方法。
Novel protein chimeras constituted of ‘silk’ and a silica-binding peptide (KSLSRHDHIHHH) were synthesized by genetic or chemical approaches and their influence on silica-silk based chimera composite formation evaluated. Genetic chimeras were constructed from 6 or 15 repeats of the 32 amino acid consensus sequence of Nephila clavipes spider silk ([SGRGGLGGQG AGAAAAAGGA GQGGYGGLGSQG]n) to which one silica binding peptide was fused at the N terminus. For the chemical chimera, 25 equivalents of the silica binding peptide were chemically coupled to natural Bombyx mori silk after modification of tyrosine groups by diazonium coupling and EDC/NHS activation of all acid groups. After silica formation under mild, biomaterial compatible conditions the effect of peptide addition on the properties of the silk and chimeric silk-silica composite materials was explored. The composite biomaterial properties could be related to the extent of silica condensation and to the higher number of silica binding sites in the chemical chimera as compared to the genetically derived variants. In all cases, the structure of the protein / chimera in solution dictated the type of composite structure that formed with the silica deposition process having little effect on the secondary structural composition of the silk based materials. Similarly to our study of genetic silk based chimeras containing the R5 peptide (SSKKSGSYSGSKGSKRRIL), the role of the chimeras (genetic and chemical) used in the present study resided more in aggregation and scaffolding than in the catalysis of condensation. The variables of peptide identity, silk construct (number of consensus repeats or silk source) and approach to synthesis (genetic or chemical) can be used to ‘tune’ the properties of the composite materials formed and is a general approach which can be used to prepare a range of materials for biomedical and sensor based applications.
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影响因子：
5.5
作者：
Grubb, DT;Jelinski, LW
通讯作者：
Jelinski, LW
DOI：
10.1111/j.1432-1033.1990.tb19354.x
发表时间：
1990-10-24
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GOORMAGHTIGH, E;CABIAUX, V;RUYSSCHAERT, JM
通讯作者：
RUYSSCHAERT, JM
影响因子：
19
作者：
Currie, Heather A.;Deschaume, Olivier;Naik, Rajesh R.;Perry, Carole C.;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
6.2
作者：
Bini, Elisabetta;Foo, Cheryl Wong Po;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
4.8
作者：
Inoue, S;Tanaka, K;Mizuno, S
通讯作者：
Mizuno, S